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Acrosin Polyclonal Antibody
Datasheet
Catalog Number:
E20-10001Amount:
100ulApplications:
WB;IHC-p;IF(paraffin section);ELISAReactivity:
Human;Rat;Mouse;Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
Synthesized peptide derived from Acrosin . at AA range: 40-120Source:
Polyclonal, Rabbit,IgG
- Western Blot analysis of various cells using Acrosin Polyclonal Antibody
Gene Name:
ACRDilution:
IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.Background:
Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids. [provided by RefSeq, Jul 2008],Protein Name:
AcrosinHuman Gene Id:
49Human Swiss Prot No:
P10323Mouse Swiss Prot No:
P23578Specificity:
Acrosin Polyclonal Antibody detects endogenous levels of Acrosin protein.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
ACR; ACRS; AcrosinObserved Band(KD):
45kDFunction:
catalytic activity:Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.,function:Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.,similarity:Belongs to the peptidase S1 family.,similarity:Contains 1 peptidase S1 domain.,subunit:Heavy chain (catalytic) and a light chain linked by two disulfide bonds.,Subcellular Location:
acrosomal vesicle,extracellular region,nucleus,Golgi-associated vesicle,acrosomal matrix,protein complex,Expression:
Leukocyte,Testis,
- Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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