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RFP-rProtein A
The protein is suitable as control reagent for RFP expression studies or as labeling reagent. Applications include: Flow cytometers, fluorescence microscopy, and microinjection, antibody purification, etc. at 1:100 dilution. Maximum absorption wavelength at 557 nm and emission wavelength at 585 nm.
Recombinant expressed in E. coli
$160/ 200μg/200μl
QuantityTotal: $160
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
BackgroundThe unique, high quality recombinant red fluorescence Protein (RFP)-rProtein A are produced in E. coli, and purified using multiple chromatographic steps (not affinity purified with human IgG). RFP-rProtein A bind to most mammalian immunoglobulins in their Fc binding domains and therefore are ideal for immunofluorescence staining. The genetically engineered protein was expressed and purified from transformed E. coli containing only the IgG binding regions and have 6X His-tag on their N-terminus. Other domains such as cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from all these proteins to ensure the maximum specific IgG binding and maximal fluorescence.
Form Or Antibody
Form of AntibodyLiquid. The product is 1 mg/ml in 10 mM Phosphate buffered saline, pH 7.4 containing, 10% glycerol and 0.1% sodium azide as preservative.
Product Information
Purity>98% by SDS-PAGE and HPLC
Endotoxin< 0.05 EU/mg
IgG Binding>95%
BioburdenNo organisms detected
Specificty Sensitivity
Specificity/SensitivityUnder optimal conditions, 1 mg RFP-rProtein A will bind approximately 5 mg IgG. Optimal binding of RFP-rProtein A to antibodies occurs at pH 5.0 to 8.0 and can be eluted over a pH range of 2.5 to 3.0.
Storage/StabilityStore at -20°C. -80°C for long-term storage. Avoid exposure to light. Avoid repeated freezing and thawing.
Price160$
Immunofluorescence staining of methanol-fixed HeLa cells (Left) and PLC-γ1 (phospho-Tyr771) Antibody (E011523) (Red).
using Synaptotagmin (Ab-309) Antibody (E021292) (Right) consequently detected with RFP-rProtein A
Conjugated Antibody
This product is also available conjugated. Format: E1RA1-020-Conjugate. Price: $485 / Size: 100μl.
E1RA1-020-FITC$485 / 100μlE1RA1-020-Biotin$485 / 100μlE1RA1-020-HRP$485 / 100μlE1RA1-020-AP$485 / 100μlE1RA1-020-AF350$485 / 100μlE1RA1-020-AF405M$485 / 100μlE1RA1-020-AF488$485 / 100μlE1RA1-020-AF514$485 / 100μlE1RA1-020-AF532$485 / 100μlE1RA1-020-AF555$485 / 100μlE1RA1-020-AF568$485 / 100μlE1RA1-020-AF405S$485 / 100μlE1RA1-020-AF405L$485 / 100μlE1RA1-020-AF546$485 / 100μlE1RA1-020-AF594$485 / 100μlE1RA1-020-AF610$485 / 100μlE1RA1-020-AF635$485 / 100μlE1RA1-020-AF647$485 / 100μlE1RA1-020-AF680$485 / 100μlE1RA1-020-AF700$485 / 100μlE1RA1-020-AF750$485 / 100μlE1RA1-020-AF790$485 / 100μlE1RA1-020-APC$485 / 100μlE1RA1-020-PE$485 / 100μlE1RA1-020-Cy3$485 / 100μlE1RA1-020-Cy5$485 / 100μlE1RA1-020-Cy5.5$485 / 100μlE1RA1-020-Cy7$485 / 100μlE1RA1-020-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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