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Secondary Antibody
Goat anti-Rabbit IgG(H+L)， PE Conjugated
FCM IF
$145/ 50Tests
QuantityTotal: $145
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Form Or Antibody
Form of AntibodyPhosphate-buffered solution, pH 7.4, containing 0.09% sodium azide
Storage/StabilityStore at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.
BackgroundAffinity purified antibody isolated from pooled antiserum from goats hyperimmunized with purified Rabbit IgG was labeled with PE. The second antibody and rabbit IgG heavy and light chain are specific reaction.
Product Information
Experimental Methods1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
3.Add 2 ml 1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add appropriate amount of fluorescent-labeled Goat anti Mouse IgG and incubate for 20 minutes away from light at room temperature.
7.Repeat step 5.
8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
[Cell fixation: 2% formaldehyde solution]
2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
3.Add 2 ml 1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add appropriate amount of fluorescent-labeled Goat anti Mouse IgG and incubate for 20 minutes away from light at room temperature.
7.Repeat step 5.
8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
[Cell fixation: 2% formaldehyde solution]
Notices1.Reagents contain NaN3. NaN3 is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately.
2.This product is for research only, not for clinical diagnosis.
2.This product is for research only, not for clinical diagnosis.
Related productsE16SPU102 Goat anti-Rabbit IgG(H+L), purity 100/500/1000μg
E16SPF102 Goat anti- Rabbit IgG(H+L), FITC conjugated 50/100 Tests
E16SPF102 Goat anti- Rabbit IgG(H+L), FITC conjugated 50/100 Tests
References1. Ledbetter, J et al. (1981) J Exp Med 153:310-323.
2. Haynes, B et al. (1986) Leukocyte Typing II: Human T lymphocytes. New York: Sprimger-Verlag:3-30.
3. Clevers H et al. (1988). Ann Rev Immunol 6: 629-662.
4. Barclay, N et al. (1993). The Leucocyte Factsbook. Academic Press. San Diego.
2. Haynes, B et al. (1986) Leukocyte Typing II: Human T lymphocytes. New York: Sprimger-Verlag:3-30.
3. Clevers H et al. (1988). Ann Rev Immunol 6: 629-662.
4. Barclay, N et al. (1993). The Leucocyte Factsbook. Academic Press. San Diego.
Price145$
Conjugated Antibody
This product is also available conjugated. Format: E16SPP102-050-Conjugate. Price: $485 / Size: 100μl.
E16SPP102-050-FITC$485 / 100μlE16SPP102-050-Biotin$485 / 100μlE16SPP102-050-HRP$485 / 100μlE16SPP102-050-AP$485 / 100μlE16SPP102-050-AF350$485 / 100μlE16SPP102-050-AF405M$485 / 100μlE16SPP102-050-AF488$485 / 100μlE16SPP102-050-AF514$485 / 100μlE16SPP102-050-AF532$485 / 100μlE16SPP102-050-AF555$485 / 100μlE16SPP102-050-AF568$485 / 100μlE16SPP102-050-AF405S$485 / 100μlE16SPP102-050-AF405L$485 / 100μlE16SPP102-050-AF546$485 / 100μlE16SPP102-050-AF594$485 / 100μlE16SPP102-050-AF610$485 / 100μlE16SPP102-050-AF635$485 / 100μlE16SPP102-050-AF647$485 / 100μlE16SPP102-050-AF680$485 / 100μlE16SPP102-050-AF700$485 / 100μlE16SPP102-050-AF750$485 / 100μlE16SPP102-050-AF790$485 / 100μlE16SPP102-050-APC$485 / 100μlE16SPP102-050-PE$485 / 100μlE16SPP102-050-Cy3$485 / 100μlE16SPP102-050-Cy5$485 / 100μlE16SPP102-050-Cy5.5$485 / 100μlE16SPP102-050-Cy7$485 / 100μlE16SPP102-050-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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