World supplier of antibodies, antigens and kits since 2007
Secondary Antibody
Goat anti-Rabbit IgG(H+L)， FITC Conjugated
FCM IF
$145/ 100μg
QuantityTotal: $145
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Form Or Antibody
Form of AntibodyPhosphate-buffered solution, pH 7.4, containing 0.09% sodium azide
Storage/StabilityStore at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.
BackgroundAffinity purified antibody isolated from pooled antiserum from goats hyperimmunized with purified Ribbit IgG was labeled with FITC. Almost no human IgG binding capacity. The green FITC (fluorescein isothiocyanate) fluorescence-labeled anti-mouse secondary antibody specific for mouse IgG molecules react with other mouse immunoglobulin light chain cross reaction. The second antibody and serum immunoglobulin without the non cross-reaction, but may be other species cross-react with immunoglobulins.
Product Information
IsotypeMouse IgG(H+L)
Experimental Methods1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
3.Add 2 ml 1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add appropriate amount of fluorescent-labeled Goat anti Mouse IgG and incubate for 20 minutes away from light at room temperature.
7.Repeat step 5.
8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
[Cell fixation: 2% formaldehyde solution]
2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
3.Add 2 ml 1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add appropriate amount of fluorescent-labeled Goat anti Mouse IgG and incubate for 20 minutes away from light at room temperature.
7.Repeat step 5.
8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
[Cell fixation: 2% formaldehyde solution]
Notices1.Reagents contain NaN3. NaN3 is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately.
2.This product is for research only, not for clinical diagnosis.
2.This product is for research only, not for clinical diagnosis.
Related productsE16SPU102 Goat anti-Rabbit IgG(H+L), purity 100/500/1000μg
E16SPP102 Goat anti- Rabbit IgG(H+L), PE conjugated 100/500μg
E16SPP101 Goat anti-Mouse IgG(H+L), PE conjugated 50/100 Tests
E16SPP102 Goat anti- Rabbit IgG(H+L), PE conjugated 100/500μg
E16SPP101 Goat anti-Mouse IgG(H+L), PE conjugated 50/100 Tests
References1. Ledbetter, J et al. (1981) J Exp Med 153:310-323.
2. Haynes, B et al. (1986) Leukocyte Typing II: Human T lymphocytes. New York: Sprimger-Verlag:3-30.
3. Clevers H et al. (1988). Ann Rev Immunol 6: 629-662.
4. Barclay, N et al. (1993). The Leucocyte Factsbook. Academic Press. San Diego.
2. Haynes, B et al. (1986) Leukocyte Typing II: Human T lymphocytes. New York: Sprimger-Verlag:3-30.
3. Clevers H et al. (1988). Ann Rev Immunol 6: 629-662.
4. Barclay, N et al. (1993). The Leucocyte Factsbook. Academic Press. San Diego.
Price145$
Conjugated Antibody
This product is also available conjugated. Format: E16SPF102-100U-Conjugate. Price: $485 / Size: 100μl.
E16SPF102-100U-FITC$485 / 100μlE16SPF102-100U-Biotin$485 / 100μlE16SPF102-100U-HRP$485 / 100μlE16SPF102-100U-AP$485 / 100μlE16SPF102-100U-AF350$485 / 100μlE16SPF102-100U-AF405M$485 / 100μlE16SPF102-100U-AF488$485 / 100μlE16SPF102-100U-AF514$485 / 100μlE16SPF102-100U-AF532$485 / 100μlE16SPF102-100U-AF555$485 / 100μlE16SPF102-100U-AF568$485 / 100μlE16SPF102-100U-AF405S$485 / 100μlE16SPF102-100U-AF405L$485 / 100μlE16SPF102-100U-AF546$485 / 100μlE16SPF102-100U-AF594$485 / 100μlE16SPF102-100U-AF610$485 / 100μlE16SPF102-100U-AF635$485 / 100μlE16SPF102-100U-AF647$485 / 100μlE16SPF102-100U-AF680$485 / 100μlE16SPF102-100U-AF700$485 / 100μlE16SPF102-100U-AF750$485 / 100μlE16SPF102-100U-AF790$485 / 100μlE16SPF102-100U-APC$485 / 100μlE16SPF102-100U-PE$485 / 100μlE16SPF102-100U-Cy3$485 / 100μlE16SPF102-100U-Cy5$485 / 100μlE16SPF102-100U-Cy5.5$485 / 100μlE16SPF102-100U-Cy7$485 / 100μlE16SPF102-100U-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
Related Products
Goat anti-Rabbit IgG SecondaryAntibody (peroxidase conjugated)E0L3012-2,$100Goat anti-Mouse IgG SecondaryAntibody (peroxidase conjugated)E0L3032-1,$60Rabbit anti-Human IgG(H&L) AntibodyE61C00201,$225Rabbit anti-Human IgG(H&L) Antibody-FITCE61C00203,$275Rabbit anti-Human IgG(H&L) Antibody-BIOTINE61C00204,$275Categories by research area
Categories by product type
- Antibodies
- Primary Antibodies
- Tag antibodies
- Internal control antibodies
- Pathological (Immunohistochemistry, IHC) antibodies
- Cell Signal Transduction Research Antibodies
- Secondary Antibody
- Antibody Sampler Kits
- ELISA Kits
- Recombinant Proteins
- Fluorescence Reagents
- Signal Inhibitors and Activators
- Transfection Reagent
- Cell biology kits
- Overexpression Cell Lines
- Cell Lysates
- Purification Resins
- 2019-nCoV & Monkeypox