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Goat anti-Mouse IgG(H+L)， FITC Conjugated
Datasheet
Catalog Number:
E16SPF101-050Amount:
50TestsForm of Antibody:
Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azideStorage/Stability:
Store at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.Background:
Affinity purified antibody isolated from pooled antiserum from goats hyperimmunized with purified mouse IgG was labeled with FITC. Almost no human IgG binding capacity. The green FITC (fluorescein isothiocyanate) fluorescence-labeled anti-mouse secondary antibody specific for mouse IgG molecules react with other mouse immunoglobulin light chain cross reaction. The second antibody and serum immunoglobulin without the non cross-reaction, but may be other species cross-react with immunoglobulins.Isotype:
Mouse IgG(H+L)Applications:
FCM IFExperimental Methods:
1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
3.Add 2 ml 1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add appropriate amount of fluorescent-labeled Goat anti Mouse IgG and incubate for 20 minutes away from light at room temperature.
7.Repeat step 5.
8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
[Cell fixation: 2% formaldehyde solution]Notices:
1.Reagents contain NaN3. NaN3 is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately.
2.This product is for research only, not for clinical diagnosis.References:
1. Ledbetter, J et al. (1981) J Exp Med 153:310-323.
2. Haynes, B et al. (1986) Leukocyte Typing II: Human T lymphocytes. New York: Sprimger-Verlag:3-30.
3. Clevers H et al. (1988). Ann Rev Immunol 6: 629-662.
4. Barclay, N et al. (1993). The Leucocyte Factsbook. Academic Press. San Diego.Related products:
E16SPU101 Goat anti-Mouse IgG(H+L), purity 100/500/1000μg
E16SPH101 Goat anti-Mouse IgG(H+L), HRPO conjugated 100/500/1000μl
E16SPB101 Goat anti-Mouse IgG(H+L), Biotin conjugated 50/100 Tests
E16SPB101 Goat anti-Mouse IgG(H+L), Biotin conjugated 100/500μg
E16SPP101 Goat anti-Mouse IgG(H+L), PE conjugated 50/100 Tests
E16SPP101 Goat anti-Mouse IgG(H+L), PE conjugated 100/500μg
Profile of Human pre-B cell line REH analyzed by flow cytometry, PE labeled goat anti-mouse IgG as secondary antibody staining.
- Human pre-B cell line REH analyzed with Purified CD10 mAb, followed by anti-mouse IgGs-PE
Price:
145$
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