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lgM Isotype Control, Purified (Biotin)
Human,Not yet tested in other species.
FC, IF, IHC,ELISA
Price on request
Quantity
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Storage/StabilityStore at 4'C. DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.
Product Information
Clone4A7C2C7C12
IsotypeMouse IgM
Storage BufferPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
DescriptionThis antibody can be used as a mouse IgM isotype control in flow cytometry and other applications. Please optimize the application conditions.
Experimental Methods(The following is a general protocol for reference only, optimization is required for best results of your experiment. )
1. Take 100μl mouse spleen cells into the bottom of 5ml tube;
2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole splenocytes,
incubate for 20 minutes at room temperature away from light;
3. Add 2 ml1xRBC lysis buffer, incubate for 10 minutes away from light after mixing,dissolve red blood cells (recommended: RBC lysing Solution 10x; Cat.: EXP016);
4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5. Add 2 ml PBS wash buffer to resuspend the cell, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be
determined on the day on the machine and can also be added fixation overnight at 4'C then measured).
[PBS wash buffer: PBS + 1% FBS +0.1% NaN3; Cat.: EXP005]
[Cell fixation: 2% formaldehyde solution]
1. Take 100μl mouse spleen cells into the bottom of 5ml tube;
2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole splenocytes,
incubate for 20 minutes at room temperature away from light;
3. Add 2 ml1xRBC lysis buffer, incubate for 10 minutes away from light after mixing,dissolve red blood cells (recommended: RBC lysing Solution 10x; Cat.: EXP016);
4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5. Add 2 ml PBS wash buffer to resuspend the cell, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be
determined on the day on the machine and can also be added fixation overnight at 4'C then measured).
[PBS wash buffer: PBS + 1% FBS +0.1% NaN3; Cat.: EXP005]
[Cell fixation: 2% formaldehyde solution]
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