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Mouse anti-Human CD69, Purified mAb
Datasheet   

  • Catalog Number:

    E16HU069-100

  • Amount:

    500Tests

  • Form of Antibody:

    Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide

  • Storage/Stability:

    Store at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.

  • Background:

    The 4AFN50 monoclonal antibody reacts with human CD69, also known as very earlyactivation antigen (VEA). CD69 is approximately 30 kDa and is expressed on the cell-surface as a disulfide-linked dimer. CD69 is rapidly upregulated upon activation and expressed on lymphocytes, monocytes and platelets.

  • Isotype:

    Mouse IgG1，kappa

  • Clone Number:

    4AFN50

  • Reactivity:

    Human,Not yet tested in other species.

  • Applications:

    FCM IF

  • Experimental Methods:

    1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
    2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
    3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
    4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
    5.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
    6.Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature.
    7.Repeat step 5.
    8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
    [PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
    [Cell fixation: 2% formaldehyde solution]

  • Notices:

    1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
    3.If the sample can not be timely analysis, please fixed;
    4.For research use only, not for diagnostic or therapeutic use.

  • References:

    1.Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
    2.Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

  • Related products:

    E16HF069 Mouse Anti-Human CD69，FITC Conjugated mAb FCM IF
    E16HP069 Mouse Anti-Human CD69, PE Conjugated mAb FCM IF
    Profile of peripheral blood lymphocytes analyzed by flow cytometry, PE labeled goat anti-mouse IgG as secondary antibody staining.

  • CD69 Purified mAb Staining of overnight PHA-stimulated normal human peripheral blood cells, followed by anti-mouse IgGs-PE

  • Price:

    300$

Add to Cart

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Catalog Number

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Mouse anti-Human CD69, Purified mAb | EnoGene Biotech Co. Ltd