Mouse anti-Human CD44, PE Conjugated mAb
Datasheet
Catalog Number:
E16HP0442-100Amount:
100TestsPrice:
255$Form of Antibody:
Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azideStorage/Stability:
Store at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.Background:
CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1(pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells.CD44 is involved in adhesion of leukocytes to endothelial cells,stromal cells and the extracellular matrix.Isotype:
Mouse IgG2aClone Number:
4AP2A1Reactivity:
Human,Not yet tested in other species.Applications:
FC IHC IP IFExperimental Methods:
1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
2.Add 10μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
[Cell fixation: 2% formaldehyde solution]Notices:
1.This reagent has been pre-diluted for use at the recommended Volume per Test.
We typically use 1 X 106 cells in a 100μl experimental sample (a test);
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
4. If the sample can not be timely analysis, please fixed;
5. For research use only, not for diagnostic or therapeutic use.References:
1. Schlossman, SL. et al. (1995) Leucocyte Typing V : White Cell Differentiation Antigens, Oxford University Press, New York. eds.
2. Gunthert U (1993) Curr.Topics Microbiol. Immunol. 184:47
3. Galandrini RE et al. (1994) J Immunol.153:21Related products:
E16HU0442 Mouse Anti-Human CD44，Purified FC IHC IP IF
E16HF0442 Mouse Anti-Human CD44, FITC Conjugated mAb FC IHC IP IF
Profile of peripheral blood lymphocytes analyzed by flow cytometry
- Human peripheral blood lymphocytes analyzed with PE CD44 mAb
Related Products
Catalog Number
- Amount:100Tests
- Price:255$
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