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Mouse anti-Human CD69, FITC Conjugated mAb
Datasheet
Catalog Number:
E16HF069-050Amount:
50TestsForm of Antibody:
Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azideStorage/Stability:
Store at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.Background:
The 4AFN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA). CD69 is approximately 30 kDa and is expressed on the cell-surface as a disulfide-linked dimer. CD69 is rapidly upregulated upon activation and expressed on lymphocytes, monocytes and platelets.Isotype:
Mouse IgG1Clone Number:
4AFN50Reactivity:
Human,Not yet tested in other species.Applications:
FCM IFExperimental Methods:
1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
2.Add 10μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
[Cell fixation: 2% formaldehyde solution]Notices:
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
3.If the sample can not be timely analysis, please fixed;
4.For research use only, not for diagnostic or therapeutic use.References:
1.Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
2.Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.Related products:
E16HU069 Mouse Anti-Human CD69，Purified mAb FCM IF
E16HP069 Mouse Anti-Human CD69, PE Conjugated mAb FCM IF
Profile of peripheral blood lymphocytes analyzed by flow cytometry
- CD69 FITC Staining of overnight PHA-stimulated normal human peripheral blood cells
Price:
145$
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