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Mouse anti-Human CD23, FITC Conjugated mAb
Datasheet   

  • Catalog Number:

    E16HF023-050

  • Amount:

    50Tests

  • Form of Antibody:

    Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide

  • Storage/Stability:

    Store at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.

  • Background:

    The 4AEBVCS2 monoclonal antibody reacts with human CD23, a 45 kDa type II transmembrane glycoprotein. CD23 is expressed on mature B cells, mantle zone B cells, follicular dendritic cells and at low levels on T, NK, langerhans cells and platelets. Expression of CD23 is upregulated upon B cell activation, and soluble forms of the antigen have been reported to be biologically active. CD23 is a low affinity receptor for IgE and is thought to play a role in the regulation of IgE response and B cell activation. CD21 and the alpha subunit of CD11b and CD11c bind to CD23.

  • Isotype:

    Mouse IgG1

  • Clone Number:

    4AEBVCS2

  • Reactivity:

    Human,Not yet tested in other species.

  • Applications:

    FCM IF

  • Experimental Methods:

    1.T Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
    2.Add 10μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
    3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
    4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
    5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
    6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
    [PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
    [Cell fixation: 2% formaldehyde solution]

  • Notices:

    1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
    3.If the sample can not be timely analysis, please fixed;
    4.For research use only, not for diagnostic or therapeutic use.

  • References:

    1.Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
    2.McMichael, A.J., P.C.L. Beverly, et al. eds. (1987). Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.
    3.Bernard, A., et al. eds. (1981). Leukocyte Typing. Springer-Verlag.

  • Related products:

    E16HF023 Mouse Anti-Human CD23，FITC Conjugated mAb FCM IF
    E16HP023 Mouse Anti-Human CD23, PE Conjugated mAb FCM IF
    E16HC023 Mouse Anti-Human CD23，PE-Cy5 Conjugated mAb FCM IF
    E16HA023 Mouse Anti-Human CD23, APC Conjugated mAb FCM IF
    E16HB023 Mouse Anti-Human CD23，Biotin Conjugated mAb FCM IF
    Profile of peripheral blood lymphocytes analyzed by flow cytometry

  • Human peripheral blood lymphocytes stained with CD23 FITC

  • Price:

    145$

Add to Cart

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Catalog Number

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Mouse anti-Human CD23, FITC Conjugated mAb | EnoGene Biotech Co. Ltd