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Mouse anti-Human CD11c, FITC Conjugated mAb
Datasheet   

  • Catalog Number:

    E16HF011c-100

  • Amount:

    100Tests

  • Form of Antibody:

    Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide

  • Storage/Stability:

    Store at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.

  • Background:

    The 4A3.9 monoclonal antibody reacts with human CD11c, the 150 kDa integrin αX chain. CD11c non-covalently associates with β2 integrin to form the CD11c/CD18 heterodimer. This complex is expressed on monocytes, granulocytes, macrophages, NK, dendritic cells and subset of T and B lymphocytes. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions.

  • Isotype:

    Mouse IgG1

  • Clone Number:

    4A3.9

  • Reactivity:

    Human,Not yet tested in other species.

  • Applications:

    FCM IF

  • Experimental Methods:

    1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
    2.Add 10μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
    3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
    4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
    5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
    6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
    [PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
    [Cell fixation: 2% formaldehyde solution]

  • Notices:

    1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
    3.If the sample can not be timely analysis, please fixed;
    4.For research use only, not for diagnostic or therapeutic use.

  • References:

    1.McMichael, A.J., P.C.L. Beverly, et al. eds. (1987). Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.
    2.Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
    3.Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

  • Related products:

    E16HU011c Mouse Anti-Human CD11c，Purified mAb FCM IF
    E16HP011c Mouse Anti-Human CD11c, PE Conjugated mAb FCM IF
    Profile of peripheral blood lymphocytes analyzed by flow cytometry

  • Human peripheral blood monoocytes analyzed with CD11c FITC mAb

  • Price:

    225$

Add to Cart

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Mouse anti-Human CD11c, FITC Conjugated mAb | EnoGene Biotech Co. Ltd