
Figure 1. Schematic diagram of the assay

500μl 500μl 500μl 500μl 500μl 500μl 1000 500 250 125 62.5 31.25 15.625 pg/ml pg/ml Figure 2. pg/ml Preparation pg/ml of MCP-1 standard pg/ml dilutions pg/ml pg/ml 4. Working solution of Biotin-Conjugate anti-human MCP-1 monoclonal antibody: Make a 1:100 dilution of the concentrated Biotin-Conjugate solution with the Biotin-Conjugate antibody Diluent in a clean plastic tube.

Figure 4. Representative standard curve for human MCP-1 ELISA. Do not use this standard curve to derive test results. A standard curve must be run for each group of microwell strips assayed. Performance Characteristics REPEATABILITY: The coefficient of variation of both intra-assay and inter-assay were less than