
Figure 1. Schematic diagram of the assay

900μl 500μl 500μl 500μl 500μl 500μl 2000 2000 1000 500 250 125 62.5 0 pg/ml pg/ml pg/ml pg/ml pg/ml pg/ml pg/ml Figure 2. Preparation of EGF standard dilutions For Research Use Only 5

Figure 4. Representative standard curve for human EGF ELISA. use this standard curve to derive test results. A standard curve must be run for microwell strips assayed.