
A Schematic diagram of the detection principle Kit components: Kit components 96T 48T 1a standard 2 Vials 1 Vial

This drawing is for reference only, and the standard curve shall be Results repeatability: Between plates, the coefficient of variation was <10%.

4. Biotinylated antibody working solution: according to each well required 100 amount, prepare 100-200 μ l more. Biotinylated antibody (2a) was diluted in dilution (2b) (1:100). It is best to prepare it when using it. 5. Enzyme conjugate working solution: dilute concentrated enzyme conjugate