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Mouse anti-Human CD38, PE-Cy5 Conjugated mAb.
Datasheet   

  • Catalog Number:

    E16HC0381-100

  • Amount:

    100Tests

  • Form of Antibody:

    Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide

  • Storage/Stability:

    Store at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.

  • Background:

    4AHIT2 reacts with CD38 antigen, a 45 kDa integral membrane glycoprotein expressed on all pre-B cells, plasma cells, thymocytes, activated T cells , NK cells, monocyte/macrophages and dentritic cells. CD38 antigen is expressed 90% of CD34+ cells, but not on pluripotent stem cells. Coexpression of CD38 + and CD34+ indicates lineage commitment of those cells. CD38 antigen acts as an ectoenzyme capable of catalysing multipe reactions and play role on regulator of cell activation and proleferation depending on cellular enviroment. Application : Leukemia phenotyping and classification; monitoring of HIV-1 infection and progression.

  • Isotype:

    Mouse IgG1

  • Clone Number:

    4AHIT2

  • Reactivity:

    Human, Not yet tested in other species.

  • Applications:

    FC IF IHC WB

  • Experimental Methods:

    1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
    2.Add 10μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
    3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
    4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
    5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
    6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
    [PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
    [Cell fixation: 2% formaldehyde solution]

  • Notices:

    1.This reagent has been pre-diluted for use at the recommended Volume per Test.
    We typically use 1 X 106 cells in a 100μl experimental sample (a test);
    2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
    4. If the sample can not be timely analysis, please fixed;
    5. For research use only, not for diagnostic or therapeutic use.

  • References:

    1. Ling NR et al., eds. (1987) Leucocyte Typing III : White Cell Differentiation Antigens, Oxford University Press, New York. 303-334.
    2. Terstappen LWMM et al., (1992) Blood 79: 666.
    3. Deaglio S et al., (2001) Leuk Res 25:1.
    4. Ghia P et al., (2003) Blood 101:1262.

  • Related products:

    E16HU0381 Mouse Anti-Human CD38, Purified mAb FC IF IHC WB
    E16HP0381 Mouse Anti-Human CD38, PE Conjugated mAb FC IF IHC WB
    E16HF0381 Mouse Anti-Human CD38, FITC Conjugated mAb FC IF IHC WB
    Profile of peripheral blood lymphocytes analyzed by flow cytometry

  • PHA and ConA activated lymphocytes analyzed with PE-Cy5 CD38 mAb

  • Price:

    255$

Add to Cart

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