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Mouse anti-Human CD34, PE-Cy5 Conjugated mAb.
Datasheet
Catalog Number:
E16HC0341-050Amount:
50TestsForm of Antibody:
Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azideStorage/Stability:
Store at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.Background:
4A43A1 reacts with CD34 molecule, a 105-120 kDa heavily O-glycosylated transmembrane glycoprotein expressed on hematopoietic progenitor cells, vascular endothelium and some tissue fibroblasts. The intracellular chain of the CD34 antigen is a target for phosphorylation by activated protein kinase C suggesting that CD34 may play a role in signal transduction. CD34 may play a role in adhesion of specific antigens to endothelium. Clone 4A43A1 belongs to the class II epitope. * CD34 mAb is useful for detection and saparation of hematopoietic stem cells.Isotype:
Mouse IgG3Clone Number:
4A43A1Reactivity:
Human,Not yet tested in other species.Applications:
FC IFExperimental Methods:
1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
2.Add 10μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
[Cell fixation: 2% formaldehyde solution]Notices:
1.This reagent has been pre-diluted for use at the recommended Volume per Test.
We typically use 1 X 106 cells in a 100μl experimental sample (a test);
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
4. If the sample can not be timely analysis, please fixed;
5. For research use only, not for diagnostic or therapeutic use.References:
1. Schlossman, SL et al., eds. (1995) Leucocyte Typing V : White Cell Differentiation Antigens, Oxford University Press, New York.
2. Freeman SD et al., (1995) Blood 85 : 2005
3. Thomas R and Lipsky PE . (1994) J Immunol 153: 4016
4.Nakamura Y. et al., ( 1994 ) Blood 83:1442Related products:
E16HU0341 Mouse Anti-Human CD34, Purified mAb FC IF
E16HF0341 Mouse Anti-Human CD34, FITC Conjugated mAb FC IF
E16HP0341 Mouse Anti-Human CD34, PE Conjugated mAb FC IF
E16HB0341 Mouse Anti-Human CD34,Biotin Conjugated mAb FC IF
Profile of peripheral blood lymphocytes analyzed by flow cytometry
- Human peripheral blood lymphocytes analyzed with CD45-FITC，CD34-PE-CY5 mAb
Price:
225$
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