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Mouse anti-Human CD45RA, Biotin Conjugated mAb.
Datasheet   

  • Catalog Number:

    E16HB0453-100/500

  • Amount:

    100Tests

  • Form of Antibody:

    Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide

  • Storage/Stability:

    Store at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.

  • Background:

    4AHI100 reacts with the 220 kDa isoform A of CD45. This is clustered as CD45RA, and is expressed on naive/resting T cells and on medullart thymocytes. In comparison, CD45RO is expressed on memory/activated T cells and cortical thymocytes. CD45RA and CD45RO are useful for discriminating between naive and memory T cells in the study of the immune system.

  • Isotype:

    Mouse IgG2b

  • Clone Number:

    4AHI100

  • Applications:

    FC IHC IP IF WB

  • Experimental Methods:

    1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
    2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
    3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
    4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
    5.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
    6.Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature.
    7.Repeat step 5.
    8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
    [PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
    [Cell fixation: 2% formaldehyde solution]

  • Notices:

    1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
    3.If the sample can not be timely analysis, please fixed;
    4.For research use only, not for diagnostic or therapeutic use.

  • References:

    1. Knapp, W et al., eds. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York.
    2. Trowbridge, IS and Thomas, ML. (1993) Annu. Rev. Immunol. 12:85.
    3. Pulido, R et al., (1988) J. Immunol. 140:3851.
    4. Roach, T et al., (1997) Curr. Biol. 7:408.

  • Related products:

    E16HU0453 Mouse Anti-Human CD45RA，Purified mAb FC IHC IP IF WB
    E16HP0453 Mouse Anti-Human CD45RA, PE Conjugated mAb FC IHC IP IF WB
    E16HF0453 Mouse Anti-Human CD45RA, FITC Conjugated mAb FC IHC IP IF WB
    Profile of peripheral blood lymphocytes analyzed by flow cytometry

  • Human peripheral blood lymphocytes analyzed with Biotin CD45RA mAb, followed by Sav-PE

  • Price:

    225$

Add to Cart

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