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Mouse anti-Human CD45, Biotin Conjugated mAb.
Datasheet   

  • Catalog Number:

    E16HB045-100/500

  • Amount:

    100/500μg

  • Form of Antibody:

    Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide and 0.2% (w/v) BSA

  • Storage/Stability:

    Store at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.

  • Background:

    4A9.4 reacts with CD45, a 180-220 kDa leukocyte common antigen (LCA). CD45 antigen is expressed at high levels on all hematopoietic cells including T and B lymphocytes, monocytes, granulocytes, NK cells and dendritic cells, but is not expressed on non-hematopoietic cells. CD45 antibody has also been reported to react weakly with mature blood erythrocytes and platelets. CD45 is a protein tyrosine phosphatase receptor that is critically important for T and B cell antigen receptor-mediated activation.*Target of immunosuppresive antibody treatment.

  • Isotype:

    Mouse IgG2a

  • Clone Number:

    4A9.4

  • Reactivity:

    Human, Not yet tested in other species.

  • Applications:

    FCM IF

  • Experimental Methods:

    1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
    2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
    3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
    4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
    5.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
    6.Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature.
    7.Repeat step 5.
    8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
    [PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
    [Cell fixation: 2% formaldehyde solution]

  • Notices:

    1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
    3.If the sample can not be timely analysis, please fixed;
    4.For research use only, not for diagnostic or therapeutic use.

  • References:

    1.Knapp, W et al., eds. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York.
    2.Trowbridge, IS and Thomas, ML. (1993) Annu. Rev. Immunol. 12:85.
    3.Pulido, R et al., (1988) J. Immunol. 140:3851.
    4.Roach, T et al., (1997) Curr. Biol. 7:408.

  • Related products:

    E16HU045 Mouse Anti-Human CD45, Purified mAb FCM IF
    E16HP045 Mouse Anti-Human CD45, PE Conjugated mAb FCM IF
    E16HC045 Mouse Anti-Human CD45, PE-Cy5 Conjugated mAb FCM IF
    E16HA045 Mouse Anti-Human CD45, APC Conjugated mAb FCM IF
    E16HF045 Mouse Anti-Human CD45, FITC Conjugated mAb FCM IF
    Profile of peripheral blood lymphocytes analyzed by flow cytometry

  • Human peripheral blood lymphocytes analyzed with Biotin CD45 mAb, followed by Sav-PE

  • Price:

    225$

Add to Cart

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