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Mouse Anti-Human CD34, Biotin Conjugated mAb.
Datasheet   

  • Catalog Number:

    E16HB0341-050/100

  • Amount:

    50Tests

  • Form of Antibody:

    Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide

  • Storage/Stability:

    Store at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.

  • Background:

    The 4A43A1 monoclonal antibody reacts with human CD34, also known as mucosialin. CD34 belongs to a protein family which also includes endoglycan and podocalyxin. Members of 4N-terminal mucin domain. CD34 was first identified as an antigen expressed on hematopoietic progenitors, and has since been extensively used as a marker to isolate cells capable of hematopoietic cell engraftment. In spite of this, the function of CD34 remains unresolved. In addition to expression on hematopoietic progenitors, CD34 is expressed on some populations of mesenchymal stem cells, tumor cell lines, and by vascular endothelia in the adult.

  • Isotype:

    Mouse IgG3

  • Clone Number:

    4A43A1

  • Applications:

    FCM IF

  • Experimental Methods:

    1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
    2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
    3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
    4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
    5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
    6.Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature.
    7.Repeat step 5.
    8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
    [PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
    [Cell fixation: 2% formaldehyde solution]

  • Notices:

    1.This reagent has been pre-diluted for use at the recommended Volume per Test.
    We typically use 1 X 106 cells in a 100μl experimental sample (a test);
    2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
    4. If the sample can not be timely analysis, please fixed;
    5. For research use only, not for diagnostic or therapeutic use.

  • References:

    1. Schlossman, SL et al., eds. (1995) Leucocyte Typing V : White Cell Differentiation Antigens, Oxford University Press, New York.
    2. Freeman SD et al., (1995) Blood 85 : 2005
    3. Thomas R and Lipsky PE . (1994) J Immunol 153: 4016
    4. Nakamura Y. et al., ( 1994 ) Blood 83:1442

  • Related products:

    E16HU0341 Mouse Anti-Human CD34, Purified mAb FCM IF
    E16HF0341 Mouse Anti-Human CD34, FITC Conjugated mAb FCM IF
    E16HP0341 Mouse Anti-Human CD34, PE Conjugated mAb FCM IF
    Profile of peripheral blood lymphocytes analyzed by flow cytometry

  • HEL cells with Biotin CD34 mAb, followed by SAV-PE

  • Price:

    145$

Add to Cart

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