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Mouse anti-Human CD19, Biotin Conjugated mAb.
Datasheet   

  • Catalog Number:

    E16HB019-100/500

  • Amount:

    100/500μg

  • Form of Antibody:

    Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide and 0.2% (w/v) BSA

  • Storage/Stability:

    Store at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.

  • Background:

    4ACB19 reacts with CD19 (B4), a 90 kDa molecule, which is expressed on approximately 5-25% of human peripheral blood lymphocytes. CD19 antigen is present on human B lymphocytes at most stages of maturation, from the earliest Ig gene rearrangement in pro-B cells to mature cell, as well as malignant B cells, but is lost on maturation to plasma cells. CD19 antibody does not react with T lymphocytes, monocytes and granulocytes. CD19 is a critical signal transduction molecule that regulates B lymphocyte development, activation and differentiation. This clone is cross reactive with non-human primate.* CD19 is a key phenotyping marker of non-T cell leukemia.

  • Isotype:

    Mouse IgG1

  • Clone Number:

    4ACB19

  • Reactivity:

    Human,Not yet tested in other species.

  • Applications:

    FCM IF

  • Experimental Methods:

    1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
    2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
    3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
    4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
    5.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
    6.Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature.
    7.Repeat step 5.
    8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
    [PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
    [Cell fixation: 2% formaldehyde solution]

  • Notices:

    1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
    3.If the sample can not be timely analysis, please fixed;
    4.For research use only, not for diagnostic or therapeutic use.

  • References:

    Nadler, LM et al. (1983) J. Immunol. 131:244
    2. Schlossman, SL et al., eds. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York.
    3. Tedder T. et al. (1994) Immunol Today. 15:437-442

  • Related products:

    E16HU019 Mouse Anti-Human CD19，Purified mAb FCM IF
    E16HP019 Mouse Anti-Human CD19, PE Conjugated mAb FCM IF
    E16HC019 Mouse Anti-Human CD19，PE-Cy5 Conjugated mAb FCM IF
    E16HA019 Mouse Anti-Human CD19, APC Conjugated mAb FCM IF
    E16HF019 Mouse Anti-Human CD19, FITC Conjugated mAb FCM IF
    Profile of peripheral blood lymphocytes analyzed by flow cytometry

  • Human peripheral blood lymphocytes analyzed with Biotin CD19 mAb, followed by Sav-PE

  • Price:

    225$

Add to Cart

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