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Mouse anti-Human CD16, Biotin Conjugated mAb.
Datasheet   

  • Catalog Number:

    E16HB016-100/500

  • Amount:

    100/500μg

  • Form of Antibody:

    Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide and 0.2% (w/v) BSA

  • Storage/Stability:

    Store at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.

  • Background:

    4ACB16 reacts with human CD16, a 50-65 kDa Fcg receptor IIIa (FcgRIII), expressed on NK cells, monocytes/macrophages and granulocytes. It is a human NK cell associated antigen. CD16 is a low affinity receptor for IgG which functions in phagocytosis and ADCC, as well as in signal transduction and NK cell activation. The CD16 antibody blocks the binding of soluble immune complexes to granulocytes.This clone is cross reactive with non-human primate.

  • Isotype:

    Mouse IgG1

  • Clone Number:

    4ACB16

  • Reactivity:

    Human,Not yet tested in other species.

  • Applications:

    FCM IF

  • Experimental Methods:

    1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
    2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
    3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
    4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
    5.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
    6.Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature.
    7.Repeat step 5.
    8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
    [PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
    [Cell fixation: 2% formaldehyde solution]

  • Notices:

    1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
    3.If the sample can not be timely analysis, please fixed;
    4.For research use only, not for diagnostic or therapeutic use.

  • References:

    1.Knapp, W.,et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York.
    2.Stroncek, D.F., et al. (1991) Blood 77:1572.
    3.Wirthmueller, U., et al. (1992) J. Exp. Med. 175:1381.
    Barclay, N.A., et al. (1993) The Leucocyte Antigen FactBook, CD16 section, Academic Press Inc.,San Diego, Calfornia, p. 136.

  • Related products:

    E16HU016 Mouse Anti-Human CD16, Purified mAb FCM IF
    E16HP016 Mouse Anti-Human CD16, PE Conjugated mAb FCM IF
    E16HA016 Mouse Anti-Human CD16, APC Conjugated mAb FCM IF
    Profile of peripheral blood lymphocytes analyzed by flow cytometry

  • Human peripheral blood lymphocytes analyzed with Biotin CD16 mAb, followed by Sav-PE

  • Price:

    225$

Add to Cart

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