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Mouse anti-Human CD15, Biotin Conjugated mAb.
Datasheet   

  • Catalog Number:

    E16HB0151-050/100

  • Amount:

    50Tests

  • Form of Antibody:

    Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide

  • Storage/Stability:

    Store at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.

  • Background:

    4AHI98 reacts with CD15 (3-FAL ), a 220 kDa carbohydrate structure, also called X-hapten. CD15 is expressed on >95% of granulocytes including neutrophils and eosinophils and to a varying degree on monodytes, but not on lymphocytes or basophils. CD15 antigen is important for direct carbohydrate-carbohydrate interaction and plays a role in mediating phagocytosis, bactericidal activity and chemotaxis.

  • Isotype:

    Mouse IgM

  • Clone Number:

    4AHI98

  • Reactivity:

    Human, Not yet tested in other species.

  • Applications:

    FC IHC ICC IF IP

  • Experimental Methods:

    1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
    2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
    3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
    4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
    5.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
    6.Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature.
    7.Repeat step 5.
    8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
    [PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
    [Cell fixation: 2% formaldehyde solution]

  • Notices:

    1.1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
    3.If the sample can not be timely analysis, please fixed;
    4.For research use only, not for diagnostic or therapeutic use.

  • References:

    1. Ledbetter, J et al. (1981) J Exp Med 153:310-323.
    2. Haynes , B et al. (1986) Leukocyte Typing II: Human T lymphocytes. New York: Sprimger-Verlag; 3-30.
    3. Clevers H et al. (1988). Ann Rev Immunol 6 : 629-662.
    4. Barclay, N et al. (1993). The Leucocyte Factsbook. Academic Press. San Diego.

  • Related products:

    E16HU0151 Mouse Anti-Human CD15, Purified mAb FC IHC ICC IF IP
    E16HP0151 Mouse Anti-Human CD15, PE Conjugated mAb FC IHC ICC IF IP
    E16HF0151 Mouse Anti-Human CD15, FITC Conjugated mAb FC IHC ICC IF IP
    Profile of peripheral blood granulocytes analyzed by flow cytometry

  • Human peripheral blood granulocytes analyzed with Biotin CD15 mAb, followed by Sav-PE

  • Price:

    145$

Add to Cart

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