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Mouse anti-Human CD14, Biotin Conjugated mAb.
Datasheet   

  • Catalog Number:

    E16HB0142-050/100

  • Amount:

    50Tests

  • Form of Antibody:

    Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide

  • Storage/Stability:

    Store at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.

  • Background:

    4AWUCD14 reacts with CD14, a 53-55 kDa molecule. CD14 is a human high affinity cell-surface receptor for complexes of lipopolysaccharide (LPS-endotoxin) and serum LPS-binding protein (LPB). CD14 antigen has a strong presence on the surface of monocytes/macrophages, is weakly expressed on granulocytes, but not expressed by myeloid progenitor cells. CD14 functions as a receptor for endotoxin; when the monocytes become activated they release cytokines such as TNF, and up-regulate cell surface molecules including adhesion molecules.This clone is cross reactive with non-human primate.

  • Isotype:

    Mouse IgG1

  • Clone Number:

    4AWUCD14

  • Reactivity:

    Human, Not yet tested in other species.

  • Applications:

    FCM IF

  • Experimental Methods:

    1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
    2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
    3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
    4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
    5.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
    6.Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature.
    7.Repeat step 5.
    8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
    [PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
    [Cell fixation: 2% formaldehyde solution]

  • Notices:

    1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
    3.If the sample can not be timely analysis, please fixed;
    4.For research use only, not for diagnostic or therapeutic use.

  • References:

    1. Knapp, W et al., eds. (1989) Leucocyte Typing IV : White Cell Differentiation Antigens, Oxford University Press, New York.
    2. Makgoba, MW et al., (1988) Nature 331: 86-88
    3. Boyd. AW et al. (1989) Blood 73: 1896-1903

  • Related products:

    E16HU0142 Mouse Anti-Human CD14, Purified mAb FCM IF
    E16HP0142 Mouse Anti-Human CD14, PE Conjugated mAb FCM IF
    E16HC0142 Mouse Anti-Human CD14, PE-Cy5 Conjugated mAb FCM IF
    E16HA0142 Mouse Anti-Human CD14, APC Conjugated mAb FCM IF
    E16HF0142 Mouse Anti-Human CD14, FITC Conjugated mAb FCM IF
    Profile of peripheral blood monocytes analyzed by flow cytometry

  • Human peripheral blood monocytes analyzed with Biotin CD14 mAb, followed by Sav-PE

  • Price:

    145$

Add to Cart

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