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Mouse anti-Human CD11b, Biotin Conjugated mAb.
Datasheet   

  • Catalog Number:

    E16HB011b--100/500

  • Amount:

    100/500μg

  • Form of Antibody:

    Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide and 0.2% (w/v) BSA

  • Storage/Stability:

    Store at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.

  • Background:

    4ALM2/1.6.11 reacts with CD11b, a member of the integrin a chain family with 165 kDa MW. which is expressed on NK cells, monocytes, granulocytes and subsets of T and B cells. It associates with CD18 to form CD11b/CD18 complex .The cellular function of CD11b is on neutrophil and monocyte interactions with stimulated endothelium; Phagocytosis of iC3b or IgG coated particles as a receptor; Chemotaxis and apoptosis.* CD11b is absent in Leukocyte Adhesion Deficiency (LAD-1) patients who have major problems with bacterial infections.This clone is cross reactive with non-human primate.

  • Isotype:

    Mouse IgG1

  • Clone Number:

    4ALM2/1.6.11

  • Reactivity:

    Human, Not yet tested in other species.

  • Applications:

    FCM IF

  • Experimental Methods:

    1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
    2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
    3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
    4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
    5.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
    6.Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature.
    7.Repeat step 5.
    8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
    [PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
    [Cell fixation: 2% formaldehyde solution]

  • Notices:

    1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
    3.If the sample can not be timely analysis, please fixed;
    4.For research use only, not for diagnostic or therapeutic use.

  • References:

    Ling NR et al., eds. (1987) Leucocyte Typing III : White Cell Differentiation Antigens, Oxford University Press, New York. 303-334
    2. Petty HR nd Todd RF. (1996) Immunol. Today 17:209
    3. Springer TA (1994) Cell 76:301
    4. Stewart M et al (1995) Curr Opin Cell Biol 7:690

  • Related products:

    E16HU011b Mouse Anti-Human CD11b, Purified mAb FCM IF
    E16HF011b Mouse Anti-Human CD11b, FITC Conjugated mAb FCM IF
    E16HP011b Mouse Anti-Human CD11b, PE Conjugated mAb FCM IF
    Profile of peripheral blood lymphocytes analyzed by flow cytometry

  • Human peripheral blood granulocytes analyzed with Biotin CD11b mAb, followed by Sav-PE

  • Price:

    225$

Add to Cart

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