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Mouse anti-Human CD8, Biotin Conjugated mAb.
Datasheet   

  • Catalog Number:

    E16HB008-100/500

  • Amount:

    100/500μg

  • Form of Antibody:

    Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide and 0.2% (w/v) BSA

  • Storage/Stability:

    Store at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.

  • Background:

    4ARiv11 reacts with the 32 kDa a chain of CD8. This molecule is expressed on the T suppressor/cytotoxic cell population (which comprises about 1/3 of the peripheral blood T lymphocytes total population) and with most of thymocytes, as well as a subset of NK cells. CD8 expresses as either a heterodimer with the CD8b chain (CD8ab) or as a homodimer (CD8aa or CD8bb). CD8 acts as a co-receptor with MHC Class I restricted TCRs in antigen recognition. CD8 function is important for positive selection of MHC Class I restricted CD8+ T cells during T cell development.* Ratio of CD4 and CD8 is important for monitor of HIV infection.

  • Isotype:

    Mouse IgG1

  • Clone Number:

    4ARiv11

  • Reactivity:

    Human, Not yet tested in other species.

  • Applications:

    FC  ICC  IHC

  • Experimental Methods:

    1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
    2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
    3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
    4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
    5.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
    6.Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature.
    7.Repeat step 5.
    8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
    [PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
    [Cell fixation: 2% formaldehyde solution]

  • Notices:

    1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
    3.If the sample can not be timely analysis, please fixed;
    4.For research use only, not for diagnostic or therapeutic use.

  • References:

    1.Engleman EG et al. (1981) J Exp Med 153: 193-198.
    2.Haynes, B et al. (1986) Leukocyte Typing II: Human T lymphocytes. New York: Sprimger-Verlag; 3-30.
    3.Parnes, JR. et al. (1989). Adv. Immunol. 44,256-311.
    4.Bikah G. et al. (1996). Science 274:1906.

  • Related products:

    E16HU008 Mouse Anti-Human CD8, Purified mAb FC  ICC  IHC
    E16HP008 Mouse Anti-Human CD8, PE Conjugated mAb FC  ICC  IHC
    E16HC008 Mouse Anti-Human CD8, PE-Cy5 Conjugated mAb FC  ICC  IHC
    E16HA008 Mouse Anti-Human CD8, APC Conjugated mAb FC  ICC  IHC
    E16HF008 Mouse Anti-Human CD8, FITC Conjugated mAb FC  ICC  IHC
    Profile of peripheral blood lymphocytes analyzed by flow cytometry

  • Human peripheral blood lymphocytes analyzed with Biotin CD8 mAb, followed by Sav-PE

  • Price:

    225$

Add to Cart

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Catalog Number

  • Amount:100/500μg
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