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Mouse anti-Human CD3, Biotin Conjugated mAb.
Datasheet   

  • Catalog Number:

    E16HB003-100/500

  • Amount:

    100/500μg

  • Form of Antibody:

    Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide and 0.2% (w/v) BSA

  • Storage/Stability:

    Store at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.

  • Background:

    4AOKT3 reacts with CD3, a 20-26 kDa molecule, which is expressed on all mature T lymphocytes (approximately 60-80% of normal human peripheral blood lymphocytes), NK-T cells and some thymocytes. CD3 associated with the T-cell receptor a/b or g/d dimer also plays a role in T-cell activation and signal transduction during antigen recognition.* CD3 mAb is used to count mature T cells in blood.

  • Isotype:

    Mouse IgG2a

  • Clone Number:

    4AOKT3

  • Reactivity:

    Human,Not yet tested in other species.

  • Applications:

    FC FA IHC  IF WB ELISA

  • Experimental Methods:

    1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
    2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
    3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
    4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
    5.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
    6.Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature.
    7.Repeat step 5.
    8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
    [PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
    [Cell fixation: 2% formaldehyde solution]

  • Notices:

    1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
    3.If the sample can not be timely analysis, please fixed;
    4.For research use only, not for diagnostic or therapeutic use.

  • References:

    Ling NR et al., eds. (1987) Leucocyte Typing III : White Cell Differentiation Antigens, Oxford University Press, New York. 303-334.
    2. Terstappen LWMM et al., (1992) Blood 79: 666.
    3. Deaglio S et al., (2001) Leuk Res 25:1.
    4. Ghia P et al., (2003) Blood 101:1262.

  • Related products:

    E16HU003 Mouse Anti-Human CD3, Purified FC FA IHC  IF WB ELISA
    E16HP003 Mouse Anti-Human CD3, PE Conjugated mAb FC FA IHC  IF WB ELISA
    E16HC003 Mouse Anti-Human CD3, PE-Cy5 Conjugated mAb FC FA IHC  IF WB ELISA
    E16HA003 Mouse Anti-Human CD3, APC Conjugated mAb FC FA IHC  IF WB ELISA
    E16HF003 Mouse Anti-Human CD3, FITC Conjugated mAb FC FA IHC  IF WB ELISA
    Profile of peripheral blood lymphocytes analyzed by flow cytometry

  • Human peripheral blood lymphocytes analyzed with Biotin CD3 mAb, followed by Sav-PE

  • Price:

    225$

Add to Cart

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