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Mouse anti-Human CD14, APC Conjugated mAb.
Datasheet
Catalog Number:
E16HA014-050Amount:
50TestsForm of Antibody:
Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide and 0.2% (w/v) BSAStorage/Stability:
Store at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.Background:
The 61D3 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14.Isotype:
Mouse IgG1Clone Number:
4A61D3Reactivity:
Human, Not yet tested in other species.Applications:
FC FA IHC IFExperimental Methods:
1. 1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
2.Add 10μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
[Cell fixation: 2% formaldehyde solution]Notices:
1.1.This reagent has been pre-diluted for use at the recommended Volume per Test.
We typically use 1 X 106 cells in a 100μl experimental sample (a test);
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
4.If the sample can not be timely analysis, please fixed;
5.For research use only, not for diagnostic or therapeutic use.References:
1. Knapp, W et al., eds. (1989) Leucocyte Typing IV : White Cell Differentiation Antigens, Oxford University Press, New York.
2. Makgoba, MW et al., (1988) Nature 331: 86-88
3. Boyd. AW et al. (1989) Blood 73: 1896-1903Related products:
E16HU014 Mouse Anti-Human CD14, Purified mAb FC FA IHC IF
E16HP014 Mouse Anti-Human CD14, PE Conjugated mAb FC FA IHC IF
E16HC014 Mouse Anti-Human CD14, PE-Cy5 Conjugated mAb FC FA IHC IF
E16HF014 Mouse Anti-Human CD14, FITC Conjugated mAb FC FA IHC IF
E16HB014 Mouse Anti-Human CD14, Biotin Conjugated mAb FC FA IHC IF
- Profile of peripheral blood monocytes analyzed by flow cytometry
Human peripheral blood monocytes analyzed with APC CD14 mAb Price:
225$
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