BackgroundVascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeabilityfactor (VPF),is a member of the PDGF family and often exists as a disulfide-linked homodimer. VEGF is expressed in a variety of tissues as multiple homodimeric forms (121, 165, 189, and 206 amino acids/monomer) resulting from alternative RNA splicing. It binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt1) and VEGF R2 (Flk1/KDR) on endothelial cells. VEGF plays a central role in tumor angiogenesis and is frequently targeted in anticancer therapies.
Product Information
Amino acid sequenceMGSSHHHHHHSSGLVPRGSHMENLYFQGMPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDLIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQQHNKCECRPKKDRARQEKCDKPRR
ActivityELISA to measure His-VEGF121 binding activity (Figure B). His-VEGF121 was immobilized on the plate and was detected by anti VEGF antibody (Santacruz sc-507).
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 1.0 mg/ml.
Shipping&StablityThe Product is shipped at ambient temperature. Upon reconstitution, the preparation is stable for up to 1 month at 2-8°C. For long term storage, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Form Or Antibody
Form of AntibodyLyophilized from a 0.22μm filtered solution at a concentration of 1mg/ml in 20mM Tris-HCl, pH8.0, 150mM NaCl.
Research AreaStem Cells Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction