All Names
AlternameMitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERK-1, p44-MAPK,p44/ Mitogen-activated protein kinase 1, MAP kinase 1, MAPK 1, ERK-2, p42-MAPK,p42
All SitesH:T202+Y204/T185+Y187, M:T203+Y205/T183+Y185, R:T203+Y205/T183+Y185
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human ERK1/2 around the phosphorylation site of threonin 202/185 and tyrosine 204/187.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityERK1/2 (Phospho-Thr202+Tyr204/Thr185+Tyr187) antibody detects endogenous levels of ERK1/2 only when phosphorylated at threonin 202/185 and tyrosine 204/187.