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Ezrin (Phospho-Tyr478) Antibody
Datasheet   

  • Catalog Number:

    E11-8031A

  • Amount:

    100μg

  • Swiss-Prot No.:

    P15311

  • Other Names:

    Cytovillin; EZRI; p81; VIL2; Villin 2

  • All Sites:

    Human: Tyr478; Mouse: Tyr478; Rat: Tyr478

  • Storage/Stability:

    Store at -20℃/1 year

  • Form of Antibody:

    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Immunogen:

    The antiserum was produced against synthesized phosphopeptide derived from human Ezrin around the phosphorylation site of tyrosine 478 (P-V-YP-E-P).

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

  • Specificity/Sensitivity:

    Ezrin (Phospho-Tyr478) antibody detects endogenous levels of Ezrin only when phosphorylated at tyrosine 478.

  • Reactivity:

    Human (Identities = 100%, Positives = 100%); Mouse (Identities = 90%, Positives = 100%);Rat (Identities = 90%, Positives = 100%)

  • Applications:

    WB: 1:500~1:1000 ELISA: 1:1000

  • References:

    Gould K.L., EMBO J. 8:4133-4142(1989).
    Turunen O., J. Biol. Chem. 264:16727-16732(1989).
    Mungall A.J., Nature 425:805-811(2003).

  • Lane 1: The extracts from K562 cells treated with Na3VO4 (0.3nM, 40mins) using Ezrin (Phospho-Tyr478) antibody
    Lane 2: The extracts from K562 cells treated with Na3VO4 (0.3nM, 40mins) plus phosphopeptide using Ezrin (Phospho-Tyr478) antibody

  • Price:

    225$

  • Research Area:

    Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

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Catalog Number

  • Amount:100μg
  • Price:225$

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