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E11-202165Productname:AIF1/IBA1PolyclonalAntibodyDatasheetCOAMSDS
Human,Rat,Mouse
WB,ELISA
Rabbit
Price on request
Quantity

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Gene NameAIF1 G1 IBA1

Product Information

Protein NameAllograft inflammatory factor 1 (AIF-1) (Ionized calcium-binding adapter molecule 1) (Protein G1)
Human Gene Id199
Human Swiss Prot NoP55008
Mouse Swiss Prot NoO70200
Rat Swiss Prot NoP55009
SpecificityAIF1 Polyclonal Antibody detects endogenous levels of protein.
FormulationLiquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 yearObserved Band : 16
Functionfunction:May play a role in macrophage activation and function.,PTM:Phosphorylated on serine residues.,similarity:Contains 2 EF-hand domains.
Subcellular Locationphagocytic cup, nucleus,cytoplasm, cytosol, actin filament, lamellipodium,rufflemembrane, perikaryon,perinuclear region of cytoplasm.
ExpressionProstate,Smooth muscle.
ImmunogenSynthesized peptide derived from part region of human protein
DilutionWB 1:500-2000 ELISA 1:5000-20000
PurificationThe antibody was affinity-purified from rabbit antiserum by affinitychromatography using epitope-specific immunogen.
Backgroundallograft inflammatory factor 1(AIF1) Homo sapiens This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, butthe full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016],
Western blot analysis of lysates from (H&L) - U2OS cells, primary antibody was diluted 1:1000(room at 1:1000, 4°overnight. 10min.
Western blot analysis of lysates from (H&L) - U2OS cells, primary antibody was diluted 1:1000(room at 1:1000, 4°overnight. 10min.
Immunofluorescence analysis of A549. 1,primary Antibody diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG Alexa Fluor 488 Secondary antibody was diluted at temperature, 50min).3, Picture B: DAPI(blue)
Immunofluorescence analysis of A549. 1,primary Antibody diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG Alexa Fluor 488 Secondary antibody was diluted at temperature, 50min).3, Picture B: DAPI(blue)