Storage/Stabilitys:stored in the dark at 4℃ and valid for one year.Notes:
1. The appropriate density of cells can increase the detection sensitivity. For 96-well plates, we recommend seeding 100 µL of cells per well, with cell concentrations ranging from 100-10,000/well for adherent cells and 2,000-50,000/well for suspension cells, with medium as blank control. For 384-well plates, both cell concentration and seeding size were halved.
2. The whole process should be aseptic, because microbial contaminants can also reduce the detection reagents and affect the experimental results.
3. Pay attention to the inoculated cell concentration and the incubation time after adding the detection reagent. Too high cell concentration or too long incubation time can lead to secondary reduction reactions, resulting in colorlessness and loss of fluorescence.
4. Avoid light when incubating.
5. This product can be detected by fluorescence or spectrophotometry, but the sensitivity of fluorescence is high and the experimental error is small. It is recommended to use fluorescence detection.