TUNEL in situ cell apoptosis detection kit, fluorescence, cell

TUNEL Apoptosis Detection Kit for adherent cells (FITC labeled POD) is one of EnoGene’s newly introduced products. It is used for detecting the fragmented DNA in the nucleus in the period of apoptosis. In this modified TUNEL assay kit, fluorescein Labeled nucleotides binds with the DNA 3´-OH ends using the natural or recombinant Terminal Deoxynucleotidyl Transferase, (TdT or rTdT).The fluorescence could be observe by fluorescence microscope. And then the compound of anti-fluorescein antibody (HRP) and HRP is bound to these fluorescein Labeled nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide and 3, 3’-Diaminobenzidine (DAB), a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.