TUNEL in situ cell apoptosis detection kit, frozen section

TUNEL Apoptosis Detection Kit for cryopreserved tissue sections (Biotin labeled POD) is one of EnoGene’s newly introduced products. It is used for detecting the fragmented DNA in the nucleus in the period of apoptosis. In this modified TUNEL assay kit, biotinylated nucleotide is labeled at the DNA 3´-OH ends using the natural or recombinant Terminal Deoxynucleotidyl Transferase, (TdT or rTdT). And then horseradish peroxidase-labeled streptavidin (Streptavidin-HRP) is bound to these biotinylated nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide and 3, 3’-Diaminobenzidine (DAB), a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.