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Perforin Polyclonal Antibody
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Product Information
Molecular Weight：61kDaCategory：Primary antibody
Recommended Dilutions：WB 1:500 - 1:2000，IHC 1:50 - 1:200，IF 1:50 - 1:200
Isotype：IgG
BufferPBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Background：The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethalautosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein.
Gene ID：5551
Immunogen：Recombinant fusion protein containing a sequence corresponding to amino acids280-555 of human Perforin (NP_001076585.1).
All Names
Altername：PRF1;FLH2;HPLH2;P1;PFN1;PFP;perforin-1;Perforin
Purification：Affinity purification
Storage/Stability：Store at -20℃. Avoid freeze / thaw cycles.
of extracts of various cell lines, using Perforin antibody antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.
Immunofluorescence analysis of NIH/3T3 cells using Perforin antibody (E90093b) at dilution of DAPI for nuclear staining.
Conjugated Antibody
This product is also available conjugated. Format: ：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-Conjugate. Price: $485 / Size: 100μl.
：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-FITC$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-Biotin$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-HRP$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-AP$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-AF350$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-AF405M$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-AF488$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-AF514$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-AF532$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-AF555$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-AF568$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-AF405S$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-AF405L$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-AF546$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-AF594$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-AF610$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-AF635$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-AF647$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-AF680$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-AF700$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-AF750$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-AF790$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-APC$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-PE$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-Cy3$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-Cy5$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-Cy5.5$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-Cy7$485 / 100μl：E90093bProductName：PerforinPolyclonalAntibodyObservedMW：70KDa-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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