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Antibody type：Primary antibody
Product Information
Conjugation：Unconjugated
Modification：Unmodification
Isotype：Rabbit IgG
Host：Rabbit
Application：WB , ICC/IF , FC , IP
Gene symbol：Np95; hNP95; ICBP90; RNF106; TDRD22; hUHRF1; huNp95
Clonality：Monoclonal antibody
Function：Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification.Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHDtype zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0),respectively, and recruits chromatin proteins.Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication.Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. May be involved in DNA repair.
Sensitivity：Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer.
Formulation：Supplied in 50nM Tris-Glycine (pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Clone Number：R06-1C4
SwissProt ID：Q96T88
Molecular Weight：Calculated MW: 89 kDa; Observed MW: 97 kDa
Purification：Affinity purifiedCross reactivity：Human , Rat
Gene Name：UHRF1
Immunogen：Recombinant protein of human UHRF1
Gene ID：29128
Storage/Stability：Store at 4°C short term.Aliquot and store at -20°C long term.Avoid freeze / thaw cycle.
Dilution：WB: 1/1000; ICC/IF: 1/50; FC: 1/20; IP: 1/20
detection of UHRF1 in K562, Hela cell lysates using UHRF1 Rabbit mAb size:89kDa. Observed band size:97kDa.
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