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GCSc-γ Polyclonal Antibody
：Human;Mouse;Rat
：WB;ELISA
Polyclonal, Rabbit,IgG
Price on request
Quantity
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Gene NameGCLC
Product Information
Protein NameGlutamate--cysteine ligase catalytic subunit
Human Gene Id2729
Human Swiss Prot NoP48506
Mouse Gene Id14629
Mouse Swiss Prot NoP97494
Rat Gene Id25283
Rat Swiss Prot NoP19468
SpecificityGCSc-γ Polyclonal Antibody detects endogenous levels of GCSc-γ protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameGCLC; GLCL; GLCLC; Glutamate--cysteine ligase catalytic subunit; GCS heavy chain; Gamma-ECS; Gamma-glutamylcysteine synthetase
Molecular Weight72766DaObserved Band:73KD
Functioncatalytic activity:ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.,disease:Defects in GCLC are the cause of hemolytic anemia [MIM:230450].,enzyme regulation:Feedback inhibition by glutathione.,pathway:Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.,similarity:Belongs to the glutamate--cysteine ligase type 3 family.,subunit:Heterodimer of a catalytic heavy chain and a regulatory light chain.,
Subcellular Locationcytosol,integral component of membrane,glutamate-cysteine ligase complex,
ExpressionForeskin fibroblast,Hippocampus,Liver,Testis,Thalamus,
ImmunogenThe antiserum was produced against synthesized peptide derived from human GCSc-gamma. AA range:266-315
DilutionWestern Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
BackgroundGlutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010],
Conjugated Antibody
This product is also available conjugated. Format: ：E20-71883-Conjugate. Price: $485 / Size: 100μl.
：E20-71883-FITC$485 / 100μl：E20-71883-Biotin$485 / 100μl：E20-71883-HRP$485 / 100μl：E20-71883-AP$485 / 100μl：E20-71883-AF350$485 / 100μl：E20-71883-AF405M$485 / 100μl：E20-71883-AF488$485 / 100μl：E20-71883-AF514$485 / 100μl：E20-71883-AF532$485 / 100μl：E20-71883-AF555$485 / 100μl：E20-71883-AF568$485 / 100μl：E20-71883-AF405S$485 / 100μl：E20-71883-AF405L$485 / 100μl：E20-71883-AF546$485 / 100μl：E20-71883-AF594$485 / 100μl：E20-71883-AF610$485 / 100μl：E20-71883-AF635$485 / 100μl：E20-71883-AF647$485 / 100μl：E20-71883-AF680$485 / 100μl：E20-71883-AF700$485 / 100μl：E20-71883-AF750$485 / 100μl：E20-71883-AF790$485 / 100μl：E20-71883-APC$485 / 100μl：E20-71883-PE$485 / 100μl：E20-71883-Cy3$485 / 100μl：E20-71883-Cy5$485 / 100μl：E20-71883-Cy5.5$485 / 100μl：E20-71883-Cy7$485 / 100μl：E20-71883-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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