BRAF35 Polyclonal Antibody
Datasheet
Catalog Number:
：E20-70515Amount:
：100ulApplications:
： WB;IHC-p;IF/ICC;ELISAReactivity:
： Human;Rat;Mouse;Gene Name:
：HMG20BProtein Name:
：SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-relatedHuman Gene Id:
：10362Human Swiss Prot No:
：Q9P0W2Mouse Swiss Prot No:
：Q9Z104Immunogen:
：The antiserum was produced against synthesized peptide derived from human HMG20B. AA range:1-50Specificity:
：BRAF35 Polyclonal Antibody detects endogenous levels of BRAF35 protein.Formulation:
：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Source:
：Polyclonal, Rabbit,IgGDilution:
：Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.Purification:
：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Concentration:
：1 mg/mlStorage Stability:
：-20°C/1 yearOther Name:
：HMG20B; BRAF35; HMGX2; HMGXB2; SMARCE1R; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related; SMARCE1-related protein; BRCA2-associated factor 35; HMG box-containing protein 20B; HMG domain-Molecular Weight:
：35813DaObserved Band：36KDBackground:
：function:Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters.,sequence caution:Translation N-terminally extended.,similarity:Contains 1 HMG box DNA-binding domain.,subcellular location:Localized to condensed chromosomes in mitosis in conjunction with BRCA2.,subunit:Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, AOF2/LSD1, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. Interacts with the BRCA2 tumor suppressor protein.,tissue specificity:Ubiquitously expressed in adult tissues.,Function:
：function:Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters.,sequence caution:Translation N-terminally extended.,similarity:Contains 1 HMG box DNA-binding domain.,subcellular location:Localized to condensed chromosomes in mitosis in conjunction with BRCA2.,subunit:Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, AOF2/LSD1, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. Interacts with the BRCA2 tumor suppressor protein.,tissue specificity:Ubiquitously expressed in adult tissues.,Subcellular Location:
：Nucleus. Chromosome. Localized to condensed chromosomes in mitosis in conjunction with BRCA2.Expression:
：Ubiquitously expressed in adult tissues.
- Western Blot analysis of various cells using BRAF35 Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit
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