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BCKDK Polyclonal Antibody
： Human;Mouse;Rat
： WB;IHC-p;IF/ICC;ELISA
：Polyclonal, Rabbit,IgG
Price on request
Quantity
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Gene Name：BCKDK
Product Information
Protein Name：[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase mitochondrial
Human Gene Id：10295
Human Swiss Prot No：O14874
Mouse Gene Id：12041
Mouse Swiss Prot No：O55028
Rat Gene Id：29603
Rat Swiss Prot No：Q00972
Specificity：BCKDK Polyclonal Antibody detects endogenous levels of BCKDK protein.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration：1 mg/ml
Storage Stability：-20°C/1 year
Other Name：BCKDK; [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase; mitochondrial; Branched-chain alpha-ketoacid dehydrogenase kinase; BCKD-kinase; BCKDHKIN
Molecular Weight：46360DaObserved Band：46KD
Function：catalytic activity:ATP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] = ADP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] phosphate.,function:Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.,PTM:Autophosphorylated.,similarity:Belongs to the PDK/BCKDK protein kinase family.,similarity:Contains 1 histidine kinase domain.,subunit:Monomer.,tissue specificity:Ubiquitous.,
Subcellular Location：Mitochondrion matrix. Mitochondrion .
Expression：Ubiquitous.
Immunogen：The antiserum was produced against synthesized peptide derived from human BCKD . AA range:11-60
Dilution：Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background：The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein encoded by this gene is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012],
Western Blot analysis of various cells using Antibody. BCKD Polyclonal Antibody. synthesized
Immunofluorescence analysis of HeLa cells, using BCKD The picture on the right is blocked with the peptide.
Conjugated Antibody
This product is also available conjugated. Format: ：E20-70467-Conjugate. Price: $485 / Size: 100μl.
：E20-70467-FITC$485 / 100μl：E20-70467-Biotin$485 / 100μl：E20-70467-HRP$485 / 100μl：E20-70467-AP$485 / 100μl：E20-70467-AF350$485 / 100μl：E20-70467-AF405M$485 / 100μl：E20-70467-AF488$485 / 100μl：E20-70467-AF514$485 / 100μl：E20-70467-AF532$485 / 100μl：E20-70467-AF555$485 / 100μl：E20-70467-AF568$485 / 100μl：E20-70467-AF405S$485 / 100μl：E20-70467-AF405L$485 / 100μl：E20-70467-AF546$485 / 100μl：E20-70467-AF594$485 / 100μl：E20-70467-AF610$485 / 100μl：E20-70467-AF635$485 / 100μl：E20-70467-AF647$485 / 100μl：E20-70467-AF680$485 / 100μl：E20-70467-AF700$485 / 100μl：E20-70467-AF750$485 / 100μl：E20-70467-AF790$485 / 100μl：E20-70467-APC$485 / 100μl：E20-70467-PE$485 / 100μl：E20-70467-Cy3$485 / 100μl：E20-70467-Cy5$485 / 100μl：E20-70467-Cy5.5$485 / 100μl：E20-70467-Cy7$485 / 100μl：E20-70467-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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