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ATP5I Polyclonal Antibody
： Human;Rat;Mouse;
：WB;IHC-p;IF(paraffin section);ELISA
：Polyclonal, Rabbit,IgG
Price on request
Quantity
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Gene Name：ATP5I
Product Information
Protein Name：ATP synthase subunit e mitochondrial
Human Gene Id：521
Human Swiss Prot No：P56385
Mouse Swiss Prot No：Q06185
Specificity：ATP5I Polyclonal Antibody detects endogenous levels of ATP5I protein.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration：1 mg/ml
Storage Stability：-20°C/1 year
Other Name：ATP5I; ATP5K; ATP synthase subunit e; mitochondrial; ATPase subunit e
Molecular Weight：7933DaObserved Band：8KD
Function：function:Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.,similarity:Belongs to the ATPase e subunit family.,subunit:F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(0) seems to have nine subunits: a, b, c,
Subcellular Location：Mitochondrion. Mitochondrion inner membrane.
Expression：Fetal brain,Kidney,
Immunogen：The antiserum was produced against synthesized peptide derived from human ATP5I. AA range:20-69
Dilution：Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background：Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the e subunit of the Fo complex. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jun 2010],
Conjugated Antibody
This product is also available conjugated. Format: ：E20-70407-Conjugate. Price: $485 / Size: 100μl.
：E20-70407-FITC$485 / 100μl：E20-70407-Biotin$485 / 100μl：E20-70407-HRP$485 / 100μl：E20-70407-AP$485 / 100μl：E20-70407-AF350$485 / 100μl：E20-70407-AF405M$485 / 100μl：E20-70407-AF488$485 / 100μl：E20-70407-AF514$485 / 100μl：E20-70407-AF532$485 / 100μl：E20-70407-AF555$485 / 100μl：E20-70407-AF568$485 / 100μl：E20-70407-AF405S$485 / 100μl：E20-70407-AF405L$485 / 100μl：E20-70407-AF546$485 / 100μl：E20-70407-AF594$485 / 100μl：E20-70407-AF610$485 / 100μl：E20-70407-AF635$485 / 100μl：E20-70407-AF647$485 / 100μl：E20-70407-AF680$485 / 100μl：E20-70407-AF700$485 / 100μl：E20-70407-AF750$485 / 100μl：E20-70407-AF790$485 / 100μl：E20-70407-APC$485 / 100μl：E20-70407-PE$485 / 100μl：E20-70407-Cy3$485 / 100μl：E20-70407-Cy5$485 / 100μl：E20-70407-Cy5.5$485 / 100μl：E20-70407-Cy7$485 / 100μl：E20-70407-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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