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ASK 1 Polyclonal Antibody
： Human;Rat;Mouse;
： WB;IHC-p;IF(paraffin section);ELISA
：Polyclonal, Rabbit,IgG
Price on request
Quantity
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Gene Name：MAP3K5
Product Information
Protein Name：Mitogen-activated protein kinase kinase kinase 5
Human Gene Id：4217
Human Swiss Prot No：Q99683
Mouse Swiss Prot No：O35099
Specificity：ASK 1 Polyclonal Antibody detects endogenous levels of ASK 1 protein.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration：1 mg/ml
Storage Stability：-20°C/1 year
Other Name：MAP3K5; ASK1; MAPKKK5; MEKK5; Mitogen-activated protein kinase kinase kinase 5; Apoptosis signal-regulating kinase 1; ASK-1; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5
Molecular Weight：154537Da
Function：catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Contains an N-terminal autoinhibitory domain. Activated by phosphorylation at Thr-838, inhibited by phosphorylation at Ser-966 and Ser-1033. Binds to, and stabilizes MAP3K6 and is activated by MAP3K6 by phosphorylation on Thr-838.,function:Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.,induction:By TNF-alpha. Inhibited by HIV-1 Nef.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Homodimer when inactive. Binds both upstream activators and dow
Subcellular Location：Cytoplasm . Endoplasmic reticulum. Interaction with 14-3-3 proteins alters the distribution of MAP3K5/ASK1 and restricts it to the perinuclear endoplasmic reticulum region.
Expression：Abundantly expressed in heart and pancreas.
Immunogen：The antiserum was produced against synthesized peptide derived from human ASK1. AA range:49-98
Dilution：Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background：Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by Re
Conjugated Antibody
This product is also available conjugated. Format: ：E20-70371-Conjugate. Price: $485 / Size: 100μl.
：E20-70371-FITC$485 / 100μl：E20-70371-Biotin$485 / 100μl：E20-70371-HRP$485 / 100μl：E20-70371-AP$485 / 100μl：E20-70371-AF350$485 / 100μl：E20-70371-AF405M$485 / 100μl：E20-70371-AF488$485 / 100μl：E20-70371-AF514$485 / 100μl：E20-70371-AF532$485 / 100μl：E20-70371-AF555$485 / 100μl：E20-70371-AF568$485 / 100μl：E20-70371-AF405S$485 / 100μl：E20-70371-AF405L$485 / 100μl：E20-70371-AF546$485 / 100μl：E20-70371-AF594$485 / 100μl：E20-70371-AF610$485 / 100μl：E20-70371-AF635$485 / 100μl：E20-70371-AF647$485 / 100μl：E20-70371-AF680$485 / 100μl：E20-70371-AF700$485 / 100μl：E20-70371-AF750$485 / 100μl：E20-70371-AF790$485 / 100μl：E20-70371-APC$485 / 100μl：E20-70371-PE$485 / 100μl：E20-70371-Cy3$485 / 100μl：E20-70371-Cy5$485 / 100μl：E20-70371-Cy5.5$485 / 100μl：E20-70371-Cy7$485 / 100μl：E20-70371-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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