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Smad3 (phospho-Ser425) Antibody

:E8RT1568DatasheetCOAMSDS
Human,Mouse,Rat
WB,IHC-p,ELISASignal Pathway:Angiogenesis; Wnt/β-Catenin; Cell Cycle
Predicted band (Da):48081
Observed band (KDa):58
Rabbit
Price on request
Quantity

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of Antibodyname:Smad3 (phospho-Ser425) Antibody
Gene NameSMAD3Protein Name:Mothers against decapentaplegic homolog 3
ImmunogenSynthesized peptide derived from human Smad3 around the phosphorylation site of S425.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificty Sensitivity

Specificity/SensitivityPhospho-Smad3 (S425) Polyclonal Antibody detects endogenous levels of Smad3 protein only when phosphorylated at S425.

Product Information

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage/Stability/ Stability:-20°C/1 yearHuman Gene ID:4088
Human Swiss-Prot No.:P84022
Mouse Gene ID:17127
Mouse Swiss-Prot No.:Q8BUN5
Rat Gene ID:25631
Rat Swiss-Prot No.:P84025
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.Other_names:SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
Western Blot analysis of various cells using Phospho-Smad3 Polyclonal Antibody diluted at 1：500.
Western Blot analysis of various cells using Phospho-Smad3 Polyclonal Antibody diluted at 1：500.
Western Blot analysis of HELA cells using Phospho-Smad3 Polyclonal Antibody diluted at 1：500.
Western Blot analysis of HELA cells using Phospho-Smad3 Polyclonal Antibody diluted at 1：500.
📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: :E8RT1568-Conjugate. Price: $485 / Size: 100μl.

:E8RT1568-FITC$485 / 100μl:E8RT1568-Biotin$485 / 100μl:E8RT1568-HRP$485 / 100μl:E8RT1568-AP$485 / 100μl:E8RT1568-AF350$485 / 100μl:E8RT1568-AF405M$485 / 100μl:E8RT1568-AF488$485 / 100μl:E8RT1568-AF514$485 / 100μl:E8RT1568-AF532$485 / 100μl:E8RT1568-AF555$485 / 100μl:E8RT1568-AF568$485 / 100μl:E8RT1568-AF405S$485 / 100μl:E8RT1568-AF405L$485 / 100μl:E8RT1568-AF546$485 / 100μl:E8RT1568-AF594$485 / 100μl:E8RT1568-AF610$485 / 100μl:E8RT1568-AF635$485 / 100μl:E8RT1568-AF647$485 / 100μl:E8RT1568-AF680$485 / 100μl:E8RT1568-AF700$485 / 100μl:E8RT1568-AF750$485 / 100μl:E8RT1568-AF790$485 / 100μl:E8RT1568-APC$485 / 100μl:E8RT1568-PE$485 / 100μl:E8RT1568-Cy3$485 / 100μl:E8RT1568-Cy5$485 / 100μl:E8RT1568-Cy5.5$485 / 100μl:E8RT1568-Cy7$485 / 100μl:E8RT1568-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.

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