| Starting From |
To |
Anti-sera |
Protein A Purified IgGs |
| |
Quantity |
QC Data |
Code |
AS |
PA |
| Peptide antigens from customers |
~ 5 mg |
>85% Pure by HPLC Correct MW by MS |
MPE |
$290
(4-12 weeks) |
|
| ~10mg |
|
$340
(4-12 weeks) |
| Protein antigens from customers |
~ 5mg |
> 90% Pure and correct MW by SDS-PAGE |
MPR |
$290
(4-12 weeks) |
|
| ~10mg |
|
$340
(4-12 weeks) |
| Peptide sequences (peptide synthesis at EnoGene) |
~15mg |
>85% Pure by HPLC Correct MW by MS |
MPS |
$490
(11-14 weeks) |
$540
(11-14 weeks) |
| Protein sequences (Peptide design and synthesis at EnoGene) |
MPD |
1. MPE-AS or MPR-AS: Basic Polyclonal Antibody Package (Mouse) 290$
| Includes |
- Conjugation to KLH (optional)
- Five Balb/c mice, pre-immune bleed, four immunizations/mouse
- ELISA test
- Antigens provided by the customer: 2.5-3 mg of peptide or 1.5 mg protein (>85% purity, 90% preferred) is required. The concentration should be >0.4 mg/ml, and the antigen solution must not contain organic solvents.
|
| Delivery |
- 10-20 µl of pre-immune serum
- ELISA results
- 1-2.5 ml of antisera from all mice
|
| Time frame |
- Conjugation: 1 week
- Immunization and ELISA: 11 weeks
- Shipping: 1 week
- Total 12-13 weeks
|
2. MPE-PA or MPR-PA: Basic Protein A-Purified Polyclonal Antibody Package (Mouse) 340$
| Includes |
- Conjugation to KLH (optional)
- 5 Balb/c mice, pre-immune bleed, 4 immunizations/mouse
- Final bleed
- Protein A purification
- ELISA test
- Antigen provided by the customer: 2.5-3 mg of peptide or 1.5 mg protein (>85% purity, 90% preferred) is required. The concentration should be >0.4 mg/ml, and no organic solvents in the antigen solution.
|
| Delivery |
- 10-20 µl of pre-immune serum
- ELISA results
- 2.5-12.5 mg protein A-purified antibodies
- Remained antigen
|
| Time frame |
- Conjugation: 1 week
- Immunization: 10 weeks
- Protein A Purification and ELISA: 1-2 week
- Shipping: 1 week
- Total 13-14 weeks
|
3. MPS-AS or MPD-AS: Complete Peptide Polyclonal Antibody Package (Mouse) 490$
| Includes |
- Peptide synthesis (≤15 a.a, >85% purity, 15 mg)
- A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
- Five Balb/c mice, pre-immune bleed, four immunizations/mouse
- ELISA test
|
| Delivery |
- 10-20 µl of pre-immune serum
- ELISA results
- 1-2.5 ml of antisera from all mice
- Remained unconjugated peptide, MS and HPLC report
|
| Time frame |
- Peptide Synthesis: 2-3 weeks
- Conjugation: 1 week
- Immunization and ELISA: 11 weeks
- Shipping: 1 week
- Total 14-16 weeks
|
4. MPS-PA or MPD-PA: Complete Protein A-Purified Peptide Polyclonal Antibody Package (Mouse)540$
| Includes |
- Peptide synthesis (≤15 a.a, >85% purity, 15 mg)
- A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
- Five Balb/C mice, pre-immune, four immunizations/mouse
- Antibody purification by protein A column
- ELISA test
|
| Delivery |
- 10-20 µl of pre-immune serum
- ELISA results
- 2.5-12.5 mg purified antibody from all antisera
- Remained unconjugated peptide, MS and HPLC report
|
| Time frame |
- Peptide Synthesis: 2-3 weeks
- Conjugation: 1 week
- Immunization: 10 weeks
- Purification and ELISA: 1-2week
- Shipping: 1 week
- Total 15-17 weeks
|
|