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Rat Custom Polyclonal Antibody Services


Starting From To Anti-sera Protein G Purified IgGs
  Quantity QC Data Code AS PG
Peptide antigens from customers ~ 5 mg >85% Pure by HPLC Correct MW by MS RPE $290 (4-12 weeks)  
~10mg   $340 (4-12 weeks)
Protein antigens from customers ~ 5mg > 90% Pure and correct MW by SDS-PAGE RPR $290 (4-12 weeks)  
~10mg   $340 (4-12 weeks)
Peptide sequences (peptide synthesis at EnoGene) ~15mg >85% Pure by HPLC Correct MW by MS RPS $490 (11-14 weeks) $540 (11-14 weeks)
Protein sequences (Peptide design and synthesis at EnoGene) RPD
     
1. RPE-AS or RPP-AS: Basic pAb Package (Rat)  290$
Includes
  • Conjugation to KLH (optional)
  • Three rats, pre-immune bleed, four immunizations/rat
  • ELISA test
  • If the antigen is provided by the customer, 2.5-3 mg of peptide or protein is required. The concentration should be >0.4 mg/ml, and the antigen solution must not contain organic solvents.
Delivery
  • 50-100 ul of pre-immune serum from each rat
  • ELISA results
  • 5-8 ml of antisera from all rats
Time frame
  • Conjugation: 1 week
  • Immunization and ELISA: 10 weeks
  • Lyophilization and shipping: 1-2 weeks
  • Total 12-13 weeks
 
2. RPE-PG or RPP-PG: Basic Protein G-Purified Polyclonal Antibody Package (Rat) 340$
Includes
  • Conjugation to KLH (optional)
  • Three rats, pre-immune bleed, 4 immunizations/rat
  • Protein G purification
  • ELISA test
  • Antigen provided by the customer: 
    2.5-3 mg of peptide or 2.5-3 mg of protein is required. The concentration should be >0.4 mg/ml, and no organic solvents in the antigen solution.
Delivery
  • 50-100 μl of pre-immune serum from each rat
  • ELISA results
  • 4.5-22.5 mg protein G-purified antibodies
  • Remained antigen
Time frame
  • Conjugation: 1 week
  • Immunization: 10 weeks
  • Protein G purification and ELISA: 1-2 week
  • shipping: 1 week
  • Total 13-14 weeks
 
3. RPS-AS or RPD-AS: Complete Peptide Polyclonal Antibody Package (Rat) 490$
Includes
  • Peptide synthesis (≤15 a.a, >85% purity, 15mg)
  • A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
  • Three rats, pre-immune bleed, four immunizations/rat
  • ELISA test
  • Guarantee Titer:>1:10,000
Delivery
  • 50-100 μl of pre-immune serum from each rat
  • ELISA results
  • 5-8 ml of antisera from all rats
  • Remained unconjugated peptide, MS and HPLC report
Time frame
  • Peptide synthesis: 2-3 weeks
  • Conjugation: 1 week
  • Immunization and ELISA: 11 weeks
  • Shipping: 1 week
  • Total 14-16 weeks
 
4. RPS-PG or RPD-PG: Complete Protein G-Purified Peptide Polyclonal Antibody Package (Rat)  540$
Includes
  • Peptide synthesis (≤15 a.a, >85% purity, 15 mg)
  • A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
  • Three rats, pre-immune, four immunizations/rat
  • Antibody purification by protein G column
  • ELISA test
  • Guarantee Titer:>1:10,000
Delivery
  • 50-100 μl of pre-immune serum from each rat
  • ELISA results
  • 4.5-22.5 mg purified antibody from all antisera
  • Remained unconjugated peptide, MS and HPLC report
Time frame
  • Peptide synthesis: 2-3 weeks
  • Conjugation: 1 week
  • Immunization: 10 weeks
  • Purification and ELISA: 1-2week
  • shipping: 1 week
  • Total 15-17 weeks