Catalog Number
|
Service Content
|
Description
|
Final Deliver
|
Time
(weeks)
|
Sub-contract Price(＄)
|
MES-PE01
|
Gene Subcloning
|
l CMV promoter based high-level expression vector
containing 6*His tag AND Factor Xa or EKrecognition site, Ready to purification and Protease enzyme cut
l Customer need to provide subclone template vector
l Preparation of the recombinant vector DNA
|
l Enzyme Digest Result
l Sequencing Map File
l 20 ug of expression-ready recombinant CMV based mammalian expression plasmid containing the gene of interest
l Detailed research report
|
～2 -2.5
|
$376/vector
(≤2kb)
If insert > 2kb, $196 additional per 1kb
|
MES-PE02
|
Gene Cloning
|
l Customer do not have template, from PCR step
l EnoGene can provide ～ 16,000 human full length cDNA cloning
l CMV promoter based high-level expression vector
containing 6*His tag AND Factor Xa or EKrecognition site, Ready to purification and Protease enzyme cut
l Customer need to provide subclone template vector
l Preparation of the recombinant vector DNA
|
l Enzyme Digest Result
l Sequencing Map File
l 20 ug of expression-ready recombinant CMV based mammalian expression plasmid containing the gene of interest
l Detailed research report
|
～2.5-3
|
$376/vector
(≤2kb)
If insert > 2kb, $196 additional per 1kb
+
$440(EnoGene cDNA cloning)
|
MES-PE03
|
Expression in target cell
|
l Transfection of recombinant vector into target cells (107 cells. Choose from 293, CHO, COS-7) for transient expression.
l Harvest and lysis of cells (usually after 2-3 days)
l Test for recombinant protein by western blot (customer provide appropriate antibodies), if desired
|
l Western Blot data
l Detailed research report
|
～2 -2.5
|
$640/target cell
|
