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E. coli Expression System Service

1. Upstream expression
 
Catalog Number
 Service Content
 Description
 Final Deliver
 Time
(weeks)
 Price(＄)
EES-PE01
 Gene Subcloning
 
     We suggest 2-3 expression vector subcloning
(Usual Expression Vector : PET, PGEX,PQE40, PRSET……)
    Customer need to provide subclone template vector
    Preparation of the recombinant vector DNA
     Enzyme Digest Result
    Sequencing Map File
    10ug Recombinant vector DNA
    Detailed research report
 ～2 -2 .5
 376/vector
(≤2kb)
If insert > 2kb, $196 additional per 1kb
EES-PE02
 Gene Cloning
     We suggest 2-3 expression vector subcloning
(PET, PGEX,PQE40, PRSET……)
    Customer do not have template, from PCR step
    EnoGene can provide ～ 16,000 human full length Cdna cloning
    Preparation of the recombinant vector DNA
    If EnoGene do not have target gene cDNA cloning, EnoGene will buy from other company or use full gene synthesis method,  the cost will be charged additionally then.
     Enzyme Digest Result
    Sequencing Map File
    10ug Recombinant vector DNA
    Detailed research report
 ～2.5-3
 376/vector
(≤2kb)
If insert > 2kb, $196 additional per 1kb
+
$440(EnoGene
cDNA cloning)
EES-PE03
 Expression Screen
     Transformation of the expression clone into different bacterial strains
    Mini-induction
    solubility test and expression control with SDS-PAGE/Coomassie stain, optionally also by Western Blotting
     Mini-induction expression SDS-PAGE/Coomassie result
    solubility test result
    Detailed research report
 ～1.5 -2
 $560/construct
+
$100/WB detection（optional）
 
 
2. Downstream expression
 
Catalog Number
 Service Content
 Description
 Final Deliver
 Time
(weeks)
 Price(＄)
EES-PE04
 Mini, middle to large scale expression
     Expression range from 100mL-100L
     Detailed research report
 ～1.5 -2.5
 100ml: $160
500ml: $240
   1L: $360
Per additional 1L, add $80, up to 10L
EES-PE05
 Protein Purification
     Protein purification using His6-tag, GST-tag, MBP-tag under native or denaturing conditions
    Identification of the purified protein by SDS-PAGE/ Coomassie stain
    Dialysis of the protein
                                                                                           
     Purified Protein Product
    Identification result of the purified protein by SDS-PAGE/ Coomassie
    Detailed research report
 ～2 -2.5
 100ml: $520
500ml: $640
   1L: $920
Per additional 1L, add $160, up to 10L
EES-PE06
  Tag and endotoxin remove
（Optional）
     Protease cut to remove tag
    Re-purification to remove Protease
    Removal of endotoxin (<1 EU/μg)
·   Inquire the quote for larger volume
     Tag removed protein
    SDS-PAGE or Western Blot data
    LAL method EU detection results
    Detailed research report
 ～2.5-3.5
 1mg: $1960
5mg: $3760
10mg: $5760