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Custom Polyclonal Antibody Services (Rat)

           Custom Polyclonal Antibody Services (Rat)

                           

 

Starting From

To

Anti-sera

Protein G Purified IgGs

 

Quantity

QC Data

Code

AS

PG

Peptide antigens from customers

~ 5 mg

>85% Pure by HPLC Correct MW by MS

RPE

Inquiry

(4-12 weeks)

 

~10mg

 

Inquiry

(4-12 weeks)

Protein antigens from customers

~ 5mg

> 90% Pure and correct MW by SDS-PAGE

RPR

Inquiry

(4-12 weeks)

 

~10mg

 

Inquiry

(4-12 weeks)

Peptide sequences (peptide synthesis at EnoGene)

~15mg

>85% Pure by HPLC Correct MW by MS

RPS

Inquiry (11-14 weeks)

Inquiry

(11-14 weeks)

Protein sequences (Peptide design and synthesis at EnoGene)

RPD

 

 

1. RPE-AS or RPP-AS: Basic pAb Package (Rat) 

Includes
  • Conjugation to KLH (optional)
  • Three rats, pre-immune bleed, four immunizations/rat
  • ELISA test
  • If the antigen is provided by the customer, 2.5-3 mg of peptide or protein is required. The concentration should be >0.4 mg/ml, and the antigen solution must not contain organic solvents.

Delivery
  • 50-100 ul of pre-immune serum from each rat
  • ELISA results
  • 5-8 ml of antisera from all rats

Time frame
  • Conjugation: 1 week
  • Immunization and ELISA: 10 weeks
  • Lyophilization and shipping: 1-2 weeks
  • Total 12-13 weeks

 

 

2. RPE-PG or RPP-PG: Basic Protein G-Purified Polyclonal Antibody Package (Rat)

Includes
  • Conjugation to KLH (optional)
  • Three rats, pre-immune bleed, 4 immunizations/rat
  • Protein G purification
  • ELISA test
  • Antigen provided by the customer:
    2.5-3 mg of peptide or 2.5-3 mg of protein is required. The concentration should be >0.4 mg/ml, and no organic solvents in the antigen solution.

Delivery
  • 50-100 μl of pre-immune serum from each rat
  • ELISA results
  • 4.5-22.5 mg protein G-purified antibodies
  • Remained antigen

Time frame
  • Conjugation: 1 week
  • Immunization: 10 weeks
  • Protein G purification and ELISA: 1-2 week
  • shipping: 1 week
  • Total 13-14 weeks

 

 

3. RPS-AS or RPD-AS: Complete Peptide Polyclonal Antibody Package (Rat)

Includes
  • Peptide synthesis (≤15 a.a, >85% purity, 15mg)
  • A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
  • Three rats, pre-immune bleed, four immunizations/rat
  • ELISA test
  • Guarantee Titer:>1:10,000

Delivery
  • 50-100 μl of pre-immune serum from each rat
  • ELISA results
  • 5-8 ml of antisera from all rats
  • Remained unconjugated peptide, MS and HPLC report

Time frame
  • Peptide synthesis: 2-3 weeks
  • Conjugation: 1 week
  • Immunization and ELISA: 11 weeks
  • Shipping: 1 week
  • Total 14-16 weeks

 

 

4. RPS-PG or RPD-PG: Complete Protein G-Purified Peptide Polyclonal Antibody Package (Rat)  

Includes
  • Peptide synthesis (≤15 a.a, >85% purity, 15 mg)
  • A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
  • Three rats, pre-immune, four immunizations/rat
  • Antibody purification by protein G column
  • ELISA test
  • Guarantee Titer:>1:10,000

Delivery
  • 50-100 μl of pre-immune serum from each rat
  • ELISA results
  • 4.5-22.5 mg purified antibody from all antisera
  • Remained unconjugated peptide, MS and HPLC report

Time frame
  • Peptide synthesis: 2-3 weeks
  • Conjugation: 1 week
  • Immunization: 10 weeks
  • Purification and ELISA: 1-2week
  • shipping: 1 week
  • Total 15-17 weeks