Custom Polyclonal Antibody Services (Rat)

Starting From
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To
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Anti-sera
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Protein G Purified IgGs
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Quantity
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QC Data
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Code
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AS
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PG
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Peptide antigens from customers
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~ 5 mg
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>85% Pure by HPLC Correct MW by MS
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RPE
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Inquiry
(4-12 weeks)
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~10mg
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Inquiry
(4-12 weeks)
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Protein antigens from customers
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~ 5mg
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> 90% Pure and correct MW by SDS-PAGE
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RPR
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Inquiry
(4-12 weeks)
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~10mg
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Inquiry
(4-12 weeks)
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Peptide sequences (peptide synthesis at EnoGene)
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~15mg
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>85% Pure by HPLC Correct MW by MS
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RPS
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Inquiry (11-14 weeks)
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Inquiry
(11-14 weeks)
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Protein sequences (Peptide design and synthesis at EnoGene)
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RPD
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1. RPE-AS or RPP-AS: Basic pAb Package (Rat)
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Includes
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- Conjugation to KLH (optional)
- Three rats, pre-immune bleed, four immunizations/rat
- ELISA test
- If the antigen is provided by the customer, 2.5-3 mg of peptide or protein is required. The concentration should be >0.4 mg/ml, and the antigen solution must not contain organic solvents.
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Delivery
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- 50-100 ul of pre-immune serum from each rat
- ELISA results
- 5-8 ml of antisera from all rats
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Time frame
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- Conjugation: 1 week
- Immunization and ELISA: 10 weeks
- Lyophilization and shipping: 1-2 weeks
- Total 12-13 weeks
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2. RPE-PG or RPP-PG: Basic Protein G-Purified Polyclonal Antibody Package (Rat)
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Includes
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- Conjugation to KLH (optional)
- Three rats, pre-immune bleed, 4 immunizations/rat
- Protein G purification
- ELISA test
- Antigen provided by the customer:
2.5-3 mg of peptide or 2.5-3 mg of protein is required. The concentration should be >0.4 mg/ml, and no organic solvents in the antigen solution.
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Delivery
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- 50-100 μl of pre-immune serum from each rat
- ELISA results
- 4.5-22.5 mg protein G-purified antibodies
- Remained antigen
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Time frame
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- Conjugation: 1 week
- Immunization: 10 weeks
- Protein G purification and ELISA: 1-2 week
- shipping: 1 week
- Total 13-14 weeks
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3. RPS-AS or RPD-AS: Complete Peptide Polyclonal Antibody Package (Rat)
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Includes
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- Peptide synthesis (≤15 a.a, >85% purity, 15mg)
- A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
- Three rats, pre-immune bleed, four immunizations/rat
- ELISA test
- Guarantee Titer:>1:10,000
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Delivery
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- 50-100 μl of pre-immune serum from each rat
- ELISA results
- 5-8 ml of antisera from all rats
- Remained unconjugated peptide, MS and HPLC report
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Time frame
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- Peptide synthesis: 2-3 weeks
- Conjugation: 1 week
- Immunization and ELISA: 11 weeks
- Shipping: 1 week
- Total 14-16 weeks
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4. RPS-PG or RPD-PG: Complete Protein G-Purified Peptide Polyclonal Antibody Package (Rat)
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Includes
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- Peptide synthesis (≤15 a.a, >85% purity, 15 mg)
- A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
- Three rats, pre-immune, four immunizations/rat
- Antibody purification by protein G column
- ELISA test
- Guarantee Titer:>1:10,000
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Delivery
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- 50-100 μl of pre-immune serum from each rat
- ELISA results
- 4.5-22.5 mg purified antibody from all antisera
- Remained unconjugated peptide, MS and HPLC report
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Time frame
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- Peptide synthesis: 2-3 weeks
- Conjugation: 1 week
- Immunization: 10 weeks
- Purification and ELISA: 1-2week
- shipping: 1 week
- Total 15-17 weeks
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