Growth Properties :adherent
Organism :Homo sapiens (human)
Source :Organ: stomach
Cell type: epithelial-like
Disease: gastric adenocarcinomar
Propagation :Complete growth medium: RPMI1640 medium, 90%; fetal bovine serum, 10%
Temperature: 37.0℃,5%CO2
Subculturing:Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37℃) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium renewal: Every 3 to 4 days
Preservation : Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: Liquid nitrogen vapor temperature