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phospho-CK18 (Ser60) Rabbit pAb
Datasheet   

  • Catalog Number:

    E47R5405

  • Amount:

    100ul

  • Cite ID#:

    EN5553576

  • Price:

    295$

  • Reactivity:

    Human,Mouse,Rat

  • Swiss-Prot No.:

    P05783

  • Form:

    Liquid

  • Altername:

    K1C18_HUMAN; Keratin, type I cytoskeletal 18; KRT18; CYK18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18 (CK-18); Keratin-18 (K18); PIG46; Keratin 18;

  • Storage/Stability:

    Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

  • Immunogen:

    KLH conjugated Synthesised phosphopeptide derived from human CK18 around the phosphorylation site of Ser60

  • Purification:

    Affinity purified by Protein A

  • Applications:

    WB:1:500-2000,IHC-P:1:100-500,IHC-F:1:100-500,ICC:1:100,IF:1:100-500,ELISA:1:5000-10000

  • Gene ID:

    3875

  • Subcellular Location:

    Cytoplasm, perinuclear region. Nucleus, nucleolus.

  • Antibody type:

    Polyclonal

  • Isotype:

    IgG

  • Immunogen range:

    MG(p-S)GG aa

  • Molecular Weight:

    48 kDa

  • Sample:
    Lane 1: Lung (Mouse) Lysate at 40 ug
    Lane 2: Uterus (Mouse) Lysate at 40 ug
    Lane 3: Small intestine (Rat) Lysate at 40 ug
    Primary: Anti-phospho-CK18 (Ser60) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 48 kD
    Observed band size: 46 kD

  • Tissue/cell:A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,) at 37°C for 20 min; Antibody incubation with (phospho-CK18 (Ser60)) polyclonal Antibody, Unconjugated 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI was used to stain the cell nuclei.

  • Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-CK18(Ser60)) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

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  • Amount:100ul
  • Price:295.00$

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