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MET Rabbit pAb
Datasheet   

  • Catalog Number:

    E47R34064
  • Amount:

    100ul
  • Cite ID#:

    EN5552956
  • Price:

    295$
  • Reactivity:

    Mouse,Rat
  • Predicted Reactivity:

    Human
  • Swiss-Prot No.:

    P08581
  • Form:

    Liquid
  • Altername:

    MET_HUMAN; Hepatocyte growth factor receptor; EC:2.7.10.1; HGF receptor; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor (SF receptor); Tyrosine-protein kinase Met; MET proto-oncogene, receptor tyrosine kinase; DA11; HGFR; AUTS9; RCCP2; c-Met; c Met; DFNB97;
  • Storage/Stability:

    Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
  • Immunogen:

    KLH conjugated synthetic peptide derived from human MET
  • Purification:

    Affinity purified by Protein A
  • Applications:

    WB:1:500-1000,IHC-P:1:100-500
  • Gene ID:

    4233
  • Subcellular Location:

    Membrane; Single-pass type I membrane protein.Isoform 3: Secreted.
  • Antibody type:

    Polyclonal
  • Isotype:

    IgG
  • Immunogen range:

    620-720/1390 aa
  • Molecular Weight:

    33/123/153 kDa
  • Sample:
    Lane 1: Mouse Kidney tissue lysates
    Lane 2: Mouse Stomach tissue lysates
    Lane 3: Mouse Lung tissue lysates
    Lane 4: Rat Kidney tissue lysates
    Lane 5: Rat Stomach tissue lysates
    Lane 6: Rat Lung tissue lysates
    Primary: Anti-MET at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 33/123/153 kDa
    Observed band size: 145 kDa
  • Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (c Met) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

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